Human LIFR/Lif R Alpha ELISA Kit PicoKine®

LIFR, also known as CD118 (Cluster of Differentiation 118), is a subunit of a receptor for leukemia inhibitory factor. This gene encodes a protein that belongs to the type I cytokine receptor family. It is mapped to 5p31.1. The LIF receptor (LIFR) is the low-affinity binding chain that, together with the high-affinity converter subunit gp130, forms a high-affinity receptor complex that mediates the action of the leukemia-inhibitory factor. LIF is a polyfunctional cytokine that affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. Mutations in this gene cause Schwartz-Jampel syndrome type 2, a disease belonging to the group of the bent-bone dysplasias. A translocation that involves the promoter of this gene, togerther with the pleiomorphic adenoma gene 1, is associated with salivary gland pleiomorphic adenoma, a common type of benign epithelial tumor of the salivary gland.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 289 279 327 287 295 18.56 6.2% Sample 2 1861 1799 1747 1540 1736 120.54 6.9% Sample 3 6421 5493 5855 5885 5913 331.13 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 289 279 327 287 295 18.56 6.2% Sample 1 289 279 327 287 295 18.56 6.2% Sample 2 1861 1799 1747 1540 1736 120.54 6.9% Sample 2 1861 1799 1747 1540 1736 120.54 6.9% Sample 3 6421 5493 5855 5885 5913 331.13 5.6% Sample 3 6421 5493 5855 5885 5913 331.13 5.6%

Cell Type Markers, Cytokines, Developmental Biology, Embryogenesis, Embryonic Stem Cells, Immunology, Innate Immunity, Intracellular, Stem Cells, Surface Molecules

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 289 1861 6421 261 1769 6236 Standard deviation 16.76 91.18 276.1 16.7 107.9 386.63 CV (%) 5.8% 4.9% 4.3% 6.4% 6.1% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 289 1861 6421 261 1769 6236 Standard deviation 16.76 91.18 276.1 16.7 107.9 386.63 CV (%) 5.8% 4.9% 4.3% 6.4% 6.1% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 289 1861 6421 261 1769 6236 Mean (pg/ml) 289 1861 6421 261 1769 6236 Standard deviation 16.76 91.18 276.1 16.7 107.9 386.63 Standard deviation 16.76 91.18 276.1 16.7 107.9 386.63 CV (%) 5.8% 4.9% 4.3% 6.4% 6.1% 6.2% CV (%) 5.8% 4.9% 4.3% 6.4% 6.1% 6.2%

Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.038 0.093 0.195 0.381 0.793 1.456 2.357 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.038 0.093 0.195 0.381 0.793 1.456 2.357 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.038 0.093 0.195 0.381 0.793 1.456 2.357 O.D. 0.008 0.038 0.093 0.195 0.381 0.793 1.456 2.357

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.