Mouse GAS6 ELISA Kit PicoKine®

GAS6 (Growth arrest-specific 6), also known as AXLLG or AXSF, is a human gene coding for the Gas6 protein. It is mapped to 13q34. GAS6 was originally found as a gene upregulated by growth arrested fibroblasts. This gene product is a gamma-carboxyglutamic acid (Gla) -containing protein which thought to be involved in the stimulation of cell proliferation, and may play a role in thrombosis. What's more, the product of the GAS6 gene, a ligand for the AXL, MER, and TYRO3 tyrosine kinase receptors, is a vitamin K-dependent protein, structurally related to anticoagulant protein S but lacking its anticoagulant activity. This protein also shows great importance in the cardiovascular system.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 50 58 58 56 55 3.27 5.9% Sample 2 232 221 213 230 224 7.58 3.3% Sample 3 1017 985 986 943 982 26.3 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 50 58 58 56 55 3.27 5.9% Sample 1 50 58 58 56 55 3.27 5.9% Sample 2 232 221 213 230 224 7.58 3.3% Sample 2 232 221 213 230 224 7.58 3.3% Sample 3 1017 985 986 943 982 26.3 2.6% Sample 3 1017 985 986 943 982 26.3 2.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 50 232 1017 51 224 949 Standard deviation 2 14.15 57.96 2.55 14.33 55.79 CV (%) 4% 6.1% 5.7% 5% 6.4% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 50 232 1017 51 224 949 Standard deviation 2 14.15 57.96 2.55 14.33 55.79 CV (%) 4% 6.1% 5.7% 5% 6.4% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 50 232 1017 51 224 949 Mean (pg/ml) 50 232 1017 51 224 949 Standard deviation 2 14.15 57.96 2.55 14.33 55.79 Standard deviation 2 14.15 57.96 2.55 14.33 55.79 CV (%) 4% 6.1% 5.7% 5% 6.4% 5.9% CV (%) 4% 6.1% 5.7% 5% 6.4% 5.9%

Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.02 0.071 0.124 0.208 0.393 0.743 1.395 2.392 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.02 0.071 0.124 0.208 0.393 0.743 1.395 2.392 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.02 0.071 0.124 0.208 0.393 0.743 1.395 2.392 O.D. 0.02 0.071 0.124 0.208 0.393 0.743 1.395 2.392

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.