Mouse MFGE8 / Lactadherin / Milk Fat Globule 1 ELISA Kit PicoKine®

Mouse MFGE8 / Lactadherin / Milk Fat Globule 1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Mfge8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, the MFGE8 gene was mapped to chromosome 15q25. MFGE8 is a factor that links apoptotic cells to phagocytes. It specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. It showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE) -dependent mechanism.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 111 112 96 112 107 6.79 6.3% Sample 2 677 586 638 627 632 32.41 5.1% Sample 3 1516 1503 1645 1622 1571 62.69 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 111 112 96 112 107 6.79 6.3% Sample 1 111 112 96 112 107 6.79 6.3% Sample 2 677 586 638 627 632 32.41 5.1% Sample 2 677 586 638 627 632 32.41 5.1% Sample 3 1516 1503 1645 1622 1571 62.69 3.9% Sample 3 1516 1503 1645 1622 1571 62.69 3.9%

Synonyms

Lactadherin; MFGM; Milk fat globule-EGF factor 8; MFG-E8; SED1; Sperm surface protein SP47; MP47; Mfge8

Gene Name

MFGE8

Gene ID

17304

UniProt

P21956

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A23-C463

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Mammary epithelial cell surfaces and spermatozoan. Isoform 2 is present in brain, heart, kidney and spleen and at low levels in lung, liver, small intestine and testis. .

Applications

ELISA

Field of Research

Angiogenesis, Apoptosis, Cancer, Cardiovascular, Cell Biology, Cell Death, Metabolism, Metabolism Processes, Pathways and Processes

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 111 677 1516 120 624 1633 Standard deviation 6.66 45.35 98.54 9 41.8 130.64 CV (%) 6% 6.7% 6.5% 7.5% 6.7% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 111 677 1516 120 624 1633 Standard deviation 6.66 45.35 98.54 9 41.8 130.64 CV (%) 6% 6.7% 6.5% 7.5% 6.7% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 111 677 1516 120 624 1633 Mean (pg/ml) 111 677 1516 120 624 1633 Standard deviation 6.66 45.35 98.54 9 41.8 130.64 Standard deviation 6.66 45.35 98.54 9 41.8 130.64 CV (%) 6% 6.7% 6.5% 7.5% 6.7% 8% CV (%) 6% 6.7% 6.5% 7.5% 6.7% 8%

Sensitivity

<10 pg/mL

Function

Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha- v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction (By similarity) . Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

51241 MW

Specificity

Natural and recombinant mouse MFGE8

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Lactadherin

Subcellular Location

Membrane ; Peripheral membrane protein . Secreted .

Sequence Similarities

Contains 2 EGF-like domains.

Protein Name

Lactadherin

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.041 0.1 0.143 0.245 0.408 0.726 1.316 2.012 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.041 0.1 0.143 0.245 0.408 0.726 1.316 2.012 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.041 0.1 0.143 0.245 0.408 0.726 1.316 2.012 O.D. 0.041 0.1 0.143 0.245 0.408 0.726 1.316 2.012

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.