Mouse Betacellulin/BTC ELISA Kit PicoKine®

Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 13 11 13 12 1 8.3% Sample 2 59 66 65 58 62 3.53 5.6% Sample 3 318 286 280 281 291 15.61 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 13 11 13 12 1 8.3% Sample 1 11 13 11 13 12 1 8.3% Sample 2 59 66 65 58 62 3.53 5.6% Sample 2 59 66 65 58 62 3.53 5.6% Sample 3 318 286 280 281 291 15.61 5.3% Sample 3 318 286 280 281 291 15.61 5.3%

Found in several mouse tissues including kidney, uterus and liver, as well as in beta tumor cell line and MCF-7 cells. It is not detected in the brain.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 59 318 10 63 326 Standard deviation 0.79 4.66 22.26 0.76 5.04 28.68 CV (%) 7.2% 7.9% 7% 7.6% 8% 8.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 59 318 10 63 326 Standard deviation 0.79 4.66 22.26 0.76 5.04 28.68 CV (%) 7.2% 7.9% 7% 7.6% 8% 8.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 11 59 318 10 63 326 Mean (pg/ml) 11 59 318 10 63 326 Standard deviation 0.79 4.66 22.26 0.76 5.04 28.68 Standard deviation 0.79 4.66 22.26 0.76 5.04 28.68 CV (%) 7.2% 7.9% 7% 7.6% 8% 8.8% CV (%) 7.2% 7.9% 7% 7.6% 8% 8.8%

Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.034 0.073 0.115 0.188 0.335 0.539 1.009 1.895 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.034 0.073 0.115 0.188 0.335 0.539 1.009 1.895 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.034 0.073 0.115 0.188 0.335 0.539 1.009 1.895 O.D. 0.034 0.073 0.115 0.188 0.335 0.539 1.009 1.895

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.