Mouse Progranulin ELISA Kit PicoKine®

Granulin, also known as Proepithelin (PEPI) is a protein that in humans is encoded by the GRN gene. This gene is mapped to 17q21.31. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2007 2106 1888 1899 1975 88.78 4.4% Sample 2 7073 7342 7311 6975 7175 155.55 2.1% Sample 3 23934 24448 24758 24233 24343 301.09 1.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2007 2106 1888 1899 1975 88.78 4.4% Sample 1 2007 2106 1888 1899 1975 88.78 4.4% Sample 2 7073 7342 7311 6975 7175 155.55 2.1% Sample 2 7073 7342 7311 6975 7175 155.55 2.1% Sample 3 23934 24448 24758 24233 24343 301.09 1.2% Sample 3 23934 24448 24758 24233 24343 301.09 1.2%

Granulins; PC cell-derived growth factor; PCDGF; Proepithelin; PEPI; Acrogranin; Progranulin; Granulin-1; Granulin-2; Granulin-3; Granulin-4; Granulin-5; Granulin-6; Granulin-7; Grn

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2007 7073 23934 1896 6992 22153 Standard deviation 130.45 339.5 1006.5%2 144.09 349.6 1019.03 CV (%) 6.5% 4.8% 4.2% 7.6% 5% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2007 7073 23934 1896 6992 22153 Standard deviation 130.45 339.5 1006.5%2 144.09 349.6 1019.03 CV (%) 6.5% 4.8% 4.2% 7.6% 5% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2007 7073 23934 1896 6992 22153 Mean (pg/ml) 2007 7073 23934 1896 6992 22153 Standard deviation 130.45 339.5 1006.5%2 144.09 349.6 1019.03 Standard deviation 130.45 339.5 1006.5%2 144.09 349.6 1019.03 CV (%) 6.5% 4.8% 4.2% 7.6% 5% 4.6% CV (%) 6.5% 4.8% 4.2% 7.6% 5% 4.6%

Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Acts as an autocrine growth factor for PC cells. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.004 0.102 0.175 0.333 0.559 1.021 1.672 2.054 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.004 0.102 0.175 0.333 0.559 1.021 1.672 2.054 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.004 0.102 0.175 0.333 0.559 1.021 1.672 2.054 O.D. 0.004 0.102 0.175 0.333 0.559 1.021 1.672 2.054

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.