Mouse SAA/SAA1 ELISA Kit PicoKine®

Serum amyloid A protein (SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 270 207 211 200 222 16.23 7.3% Sample 2 1129 1020 856 655 915 32.12 3.5% Sample 3 2924 4991 2925 4112 3738 240.73 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 270 207 211 200 222 16.23 7.3% Sample 1 270 207 211 200 222 16.23 7.3% Sample 2 1129 1020 856 655 915 32.12 3.5% Sample 2 1129 1020 856 655 915 32.12 3.5% Sample 3 2924 4991 2925 4112 3738 240.73 6.4% Sample 3 2924 4991 2925 4112 3738 240.73 6.4%

Cardiovascular, Complement, Cytokines, G Protein Signaling, Immunology, Innate Immunity, Lipids/Lipoproteins, Macrophage/Inflammation, Signal Transduction, Signaling Pathway

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 270 1129 2924 194 1044 3393 Standard deviation 9.26 70.9 166.67 9.72 52.93 245.81 CV (%) 3.4% 6.3% 5.7% 5% 5.1% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 270 1129 2924 194 1044 3393 Standard deviation 9.26 70.9 166.67 9.72 52.93 245.81 CV (%) 3.4% 6.3% 5.7% 5% 5.1% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 270 1129 2924 194 1044 3393 Mean (pg/ml) 270 1129 2924 194 1044 3393 Standard deviation 9.26 70.9 166.67 9.72 52.93 245.81 Standard deviation 9.26 70.9 166.67 9.72 52.93 245.81 CV (%) 3.4% 6.3% 5.7% 5% 5.1% 7.5% CV (%) 3.4% 6.3% 5.7% 5% 5.1% 7.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.093 0.175 0.322 0.578 0.971 1.609 1.938 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.093 0.175 0.322 0.578 0.971 1.609 1.938 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.001 0.093 0.175 0.322 0.578 0.971 1.609 1.938 O.D. 0.001 0.093 0.175 0.322 0.578 0.971 1.609 1.938

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.