Mouse MSP/MST1 ELISA Kit PicoKine®

Macrophage Stimulating 1 (SMT1), also known as Hepatocyte growth factor-like protein (HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3 (3p21) . The RON tyrosine kinase), the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 257 283 261 264 266 9.98 3.7% Sample 2 1513 1499 1287 1336 1408 98.9 7% Sample 3 5860 5664 5841 5939 5826 100.49 1.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 257 283 261 264 266 9.98 3.7% Sample 1 257 283 261 264 266 9.98 3.7% Sample 2 1513 1499 1287 1336 1408 98.9 7% Sample 2 1513 1499 1287 1336 1408 98.9 7% Sample 3 5860 5664 5841 5939 5826 100.49 1.7% Sample 3 5860 5664 5841 5939 5826 100.49 1.7%

Hepatocyte growth factor-like protein; Macrophage stimulatory protein; MSP; Hepatocyte growth factor-like protein alpha chain; Hepatocyte growth factor-like protein beta chain; Mst1; Hgfl

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 257 1513 5860 276 1456 5996 Standard deviation 11.56 62.03 322.3 12.97 61.15 425.71 CV (%) 4.5% 4.1% 5.5% 4.7% 4.2% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 257 1513 5860 276 1456 5996 Standard deviation 11.56 62.03 322.3 12.97 61.15 425.71 CV (%) 4.5% 4.1% 5.5% 4.7% 4.2% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 257 1513 5860 276 1456 5996 Mean (pg/ml) 257 1513 5860 276 1456 5996 Standard deviation 11.56 62.03 322.3 12.97 61.15 425.71 Standard deviation 11.56 62.03 322.3 12.97 61.15 425.71 CV (%) 4.5% 4.1% 5.5% 4.7% 4.2% 7.1% CV (%) 4.5% 4.1% 5.5% 4.7% 4.2% 7.1%

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation. Phosphorylates MOBKL1A, MOBKL1B and RASSF2. Phosphorylates TNNI3 (cardiac Tn-I) and alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T) . Phosphorylates FOXO1 on 'Ser-212' and regulates its activation and stimulates transcription of PMAIP1 in a FOXO1-dependent manner. Phosphorylates SIRT1 and inhibits SIRT1-mediated p53/TP53 deacetylation, thereby promoting p53/TP53 dependent transcription and apoptosis upon DNA damage. Acts as an inhibitor of PKB/AKT1. Phosphorylates AR on 'Ser-650' and suppresses its activity by intersecting with PKB/AKT1 signaling and antagonizing formation of AR-chromatin complexes (By similarity) .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.135 0.233 0.445 0.857 1.343 1.913 2.017 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.135 0.233 0.445 0.857 1.343 1.913 2.017 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.135 0.233 0.445 0.857 1.343 1.913 2.017 O.D. 0.017 0.135 0.233 0.445 0.857 1.343 1.913 2.017

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.