Mouse ACE2 ELISA Kit PicoKine®

Mouse ACE2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Ace2 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

AngiotensinI-converting enzyme 2 (ACE 2) is a protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. By sequence similarity to a sequence in GenBank, this gene is mapped to Xp22.2. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 146 150 152 149 149 2.16 1.4% Sample 2 538 544 526 551 539 9.17 1.7% Sample 3 1252 1287 1257 1185 1245 37.27 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 146 150 152 149 149 2.16 1.4% Sample 1 146 150 152 149 149 2.16 1.4% Sample 2 538 544 526 551 539 9.17 1.7% Sample 2 538 544 526 551 539 9.17 1.7% Sample 3 1252 1287 1257 1185 1245 37.27 2.9% Sample 3 1252 1287 1257 1185 1245 37.27 2.9%

Synonyms

Angiotensin-converting enzyme 2;3.4.17.23; ACE-related carboxypeptidase; Processed angiotensin-converting enzyme 2; Ace2

Gene Name

ACE2

Gene ID

70008

UniProt

Q8R0I0

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q18-T740

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in heart, kidney and forebrain (at protein level) . Ubiquitously expressed, with highest levels in ileum, kidney and lung. In lung, expressed on vascular endothelial and airway epithelial cells. .

Applications

ELISA

Field of Research

Cell Biology, Cardiovascular, Proteolysis/Ubiquitin, Contractility, Vasculature, Metalloprotease, Proteolytic Enzymes

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 146 538 1252 131 557 1192 Standard deviation 11.38 27.88 75.77 10.48 31.74 84.63 CV (%) 7.8% 4.7% 6.1% 8% 5.7% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 146 538 1252 131 557 1192 Standard deviation 11.38 27.88 75.77 10.48 31.74 84.63 CV (%) 7.8% 4.7% 6.1% 8% 5.7% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 146 538 1252 131 557 1192 Mean (pg/ml) 146 538 1252 131 557 1192 Standard deviation 11.38 27.88 75.77 10.48 31.74 84.63 Standard deviation 11.38 27.88 75.77 10.48 31.74 84.63 CV (%) 7.8% 4.7% 6.1% 8% 5.7% 7.1% CV (%) 7.8% 4.7% 6.1% 8% 5.7% 7.1%

Sensitivity

<10 pg/mL

Function

Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin- 13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. May have a protective role in acute lung injury. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

92368 MW

Specificity

Natural and recombinant mouse ACE2

Anticoagulant

Heparin

Gene Name Synonym

Angiotensin-converting enzyme 2

Subcellular Location

Processed angiotensin-converting enzyme 2: Secreted .

Protein Name

Angiotensin-converting enzyme 2

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.061 0.161 0.244 0.428 0.737 1.179 1.792 2.225 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.061 0.161 0.244 0.428 0.737 1.179 1.792 2.225 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.061 0.161 0.244 0.428 0.737 1.179 1.792 2.225 O.D. 0.061 0.161 0.244 0.428 0.737 1.179 1.792 2.225

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.