Mouse Periostin/OSF2 ELISA Kit PicoKine®

Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/ beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 109 109 111 107 109 1.41 1.2% Sample 2 795 840 784 883 825 39.27 4.7% Sample 3 2510 2434 2494 2308 2436 79.41 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 109 109 111 107 109 1.41 1.2% Sample 1 109 109 111 107 109 1.41 1.2% Sample 2 795 840 784 883 825 39.27 4.7% Sample 2 795 840 784 883 825 39.27 4.7% Sample 3 2510 2434 2494 2308 2436 79.41 3.2% Sample 3 2510 2434 2494 2308 2436 79.41 3.2%

Atherosclerosis, Cancer, Cardiogenesis, Cardiovascular, Cell Adhesion Molecules, Cell Type Markers, Cytoskeleton/ECM, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction, Structures, Tags & Cell Markers, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 109 7119 2510 119 740 2598 Standard deviation 6.97 53.26 110.44 8.33 62.9 119.5 CV (%) 6.4% 6.7% 4.4% 7% 8.5% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 109 7119 2510 119 740 2598 Standard deviation 6.97 53.26 110.44 8.33 62.9 119.5 CV (%) 6.4% 6.7% 4.4% 7% 8.5% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 109 7119 2510 119 740 2598 Mean (pg/ml) 109 7119 2510 119 740 2598 Standard deviation 6.97 53.26 110.44 8.33 62.9 119.5 Standard deviation 6.97 53.26 110.44 8.33 62.9 119.5 CV (%) 6.4% 6.7% 4.4% 7% 8.5% 4.6% CV (%) 6.4% 6.7% 4.4% 7% 8.5% 4.6%

Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.043 0.099 0.152 0.269 0.454 0.833 1.44 2.129 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.043 0.099 0.152 0.269 0.454 0.833 1.44 2.129 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.043 0.099 0.152 0.269 0.454 0.833 1.44 2.129 O.D. 0.043 0.099 0.152 0.269 0.454 0.833 1.44 2.129

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.