Mouse SP-D ELISA Kit PicoKine®

Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What's more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 641 579 553 633 601 36.77 6.1% Sample 2 2014 2168 2058 1954 2048 78.24 3.8% Sample 3 9766 8221 8235 9714 8984 756.23 8.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 641 579 553 633 601 36.77 6.1% Sample 1 641 579 553 633 601 36.77 6.1% Sample 2 2014 2168 2058 1954 2048 78.24 3.8% Sample 2 2014 2168 2058 1954 2048 78.24 3.8% Sample 3 9766 8221 8235 9714 8984 756.23 8.4% Sample 3 9766 8221 8235 9714 8984 756.23 8.4%

Cancer Metabolism, Cell Biology, Defensin, Human Protein, Immunology, Innate Immunity, Macrophage/Inflammation, Metabolism, Microbiology, Oxidative Stress, Pathways and Processes, Protein, Redox Metabolism, Signal Transduction, Signaling Pathway

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 641 2014 9766 618 1957 9758 Standard deviation 26.92 151.05 625.02 37.69 162.43 692.81 CV (%) 4.2% 7.5% 6.4% 6.1% 8.3% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 641 2014 9766 618 1957 9758 Standard deviation 26.92 151.05 625.02 37.69 162.43 692.81 CV (%) 4.2% 7.5% 6.4% 6.1% 8.3% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 641 2014 9766 618 1957 9758 Mean (pg/ml) 641 2014 9766 618 1957 9758 Standard deviation 26.92 151.05 625.02 37.69 162.43 692.81 Standard deviation 26.92 151.05 625.02 37.69 162.43 692.81 CV (%) 4.2% 7.5% 6.4% 6.1% 8.3% 7.1% CV (%) 4.2% 7.5% 6.4% 6.1% 8.3% 7.1%

Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.01 0.085 0.155 0.258 0.467 0.824 1.321 2.076 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.01 0.085 0.155 0.258 0.467 0.824 1.321 2.076 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.01 0.085 0.155 0.258 0.467 0.824 1.321 2.076 O.D. 0.01 0.085 0.155 0.258 0.467 0.824 1.321 2.076

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.