Mouse IGFBP5 ELISA Kit PicoKine®

Mouse IGFBP5 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Igfbp5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The IGFBP5 gene was mapped to chromosome 2q33-q34. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection was inhibitory to growth in 2 human breast cancer cell lines. Stable expression of IGFBP5 in the breast cancer cell lines also inhibited the formation and growth of tumors following injection in athymic mice. IGFBP5 is believed a growth inhibitor and proapoptotic agent in breast cancer cells.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 223 257 257 258 248 14.87 5.9% Sample 2 1270 1408 1312 1242 1308 62.88 4.8% Sample 3 6078 5394 5607 6353 5858 378.06 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 223 257 257 258 248 14.87 5.9% Sample 1 223 257 257 258 248 14.87 5.9% Sample 2 1270 1408 1312 1242 1308 62.88 4.8% Sample 2 1270 1408 1312 1242 1308 62.88 4.8% Sample 3 6078 5394 5607 6353 5858 378.06 6.4% Sample 3 6078 5394 5607 6353 5858 378.06 6.4%

Synonyms

Insulin-like growth factor-binding protein 5; IBP-5; IGF-binding protein 5; IGFBP-5; Igfbp5; Igfbp-5

Gene Name

IGFBP5

Gene ID

16011

UniProt

Q07079

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-E271

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Most abundant in kidney, uterus and gastrocnemius muscle.

Applications

ELISA

Field of Research

Growth Factors/Hormones, Lipid Signaling, Signal Transduction, Signaling Pathway

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 223 1270 6078 229 1195 6403 Standard deviation 16.5 71.12 257.4%7 21.29 76.48 332.95 CV (%) 7.4% 5.6% 4.2% 9.3% 6.4% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 223 1270 6078 229 1195 6403 Standard deviation 16.5 71.12 257.4%7 21.29 76.48 332.95 CV (%) 7.4% 5.6% 4.2% 9.3% 6.4% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 223 1270 6078 229 1195 6403 Mean (pg/ml) 223 1270 6078 229 1195 6403 Standard deviation 16.5 71.12 257.4%7 21.29 76.48 332.95 Standard deviation 16.5 71.12 257.4%7 21.29 76.48 332.95 CV (%) 7.4% 5.6% 4.2% 9.3% 6.4% 5.2% CV (%) 7.4% 5.6% 4.2% 9.3% 6.4% 5.2%

Sensitivity

<10 pg/mL

Function

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

30372 MW

Specificity

Natural and recombinant mouse IGFBP5

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Insulin-like growth factor-binding protein 5

Subcellular Location

Secreted.

Sequence Similarities

Contains 1 IGFBP N-terminal domain.

Protein Name

Insulin-like growth factor-binding protein 5

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.065 0.11 0.195 0.369 0.673 1.143 1.929 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.065 0.11 0.195 0.369 0.673 1.143 1.929 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.065 0.11 0.195 0.369 0.673 1.143 1.929 O.D. 0.002 0.065 0.11 0.195 0.369 0.673 1.143 1.929

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.