Mouse IL-17RC ELISA Kit PicoKine®

Interleukin-17 receptor C is a protein that in humans is encoded by the IL17RC gene. By genomic sequence analysis, IL17RC gene was mapped to chromosome 3p25.3-p24.1. This gene encodes a single-pass transmembrane protein that shares limited similarity with the interleukin-17 receptor. Multiple alternatively spliced transcript variants encoding different isoforms have been detected for this gene, but the full-length nature of only three have been determined to date. The biologic activity of IL-17 is dependent on a complex composed of IL-17RA and IL-17RC, suggesting a new paradigm for understanding the interactions between the expanded family of IL-17 ligands and their receptors.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 184 193 217 202 199 12.18 6.1% Sample 2 1503 1674 1723 1753 1663 96.72 5.8% Sample 3 5668 5483 6424 5529 5776 380.27 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 184 193 217 202 199 12.18 6.1% Sample 1 184 193 217 202 199 12.18 6.1% Sample 2 1503 1674 1723 1753 1663 96.72 5.8% Sample 2 1503 1674 1723 1753 1663 96.72 5.8% Sample 3 5668 5483 6424 5529 5776 380.27 6.5% Sample 3 5668 5483 6424 5529 5776 380.27 6.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 184 1503 5668 190 1560 5820 Standard deviation 11.77 60.12 345.74 13.68 65.52 413.22 CV (%) 6.4% 4% 6.1% 7.2% 4.2% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 184 1503 5668 190 1560 5820 Standard deviation 11.77 60.12 345.74 13.68 65.52 413.22 CV (%) 6.4% 4% 6.1% 7.2% 4.2% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 184 1503 5668 190 1560 5820 Mean (pg/ml) 184 1503 5668 190 1560 5820 Standard deviation 11.77 60.12 345.74 13.68 65.52 413.22 Standard deviation 11.77 60.12 345.74 13.68 65.52 413.22 CV (%) 6.4% 4% 6.1% 7.2% 4.2% 7.1% CV (%) 6.4% 4% 6.1% 7.2% 4.2% 7.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.074 0.134 0.248 0.484 0.803 1.355 1.936 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.074 0.134 0.248 0.484 0.803 1.355 1.936 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.074 0.134 0.248 0.484 0.803 1.355 1.936 O.D. 0.006 0.074 0.134 0.248 0.484 0.803 1.355 1.936

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.