Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine®

Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Il1rl1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin 1 receptor-like 1, also known as IL1RL1 or ST2, is a protein that in humans is encoded by the IL1RL1 gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. TL1RL1 is necessary for endotoxin tolerance and, by inhibiting TLR responses, enhances Th2 responses. This gene, interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2) and interleukin 1 receptor-like 2 (IL1RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 194 192 199 206 197 5.4 2.7% Sample 2 1525 1366 1502 1386 1444 69.58 4.8% Sample 3 5484 5267 4842 5202 5198 230.92 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 194 192 199 206 197 5.4 2.7% Sample 1 194 192 199 206 197 5.4 2.7% Sample 2 1525 1366 1502 1386 1444 69.58 4.8% Sample 2 1525 1366 1502 1386 1444 69.58 4.8% Sample 3 5484 5267 4842 5202 5198 230.92 4.4% Sample 3 5484 5267 4842 5202 5198 230.92 4.4%

Synonyms

Interleukin-1 receptor-like 1; Lymphocyte antigen 84; Protein ST2; Protein T1; Il1rl1; Ly84, St2, Ste2

Gene Name

IL1RL1

Gene ID

17082

UniProt

P14719

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S27-R332

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Predominantly expressed in hematopoietic tissues, and in macrophage, erythroid, epithelial and fibroblast cell lines. .

Applications

ELISA

Field of Research

Angiogenesis, Cardiovascular, Cytokines, Hypertrophy, Immunology, Innate Immunity, Interleukins, TLR Signaling

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 194 1525 5484 189 1538 5883 Standard deviation 8.73 94.55 296.13 10.77 115.35 394.16 CV (%) 4.5% 6.2% 5.4% 5.7% 7.5% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 194 1525 5484 189 1538 5883 Standard deviation 8.73 94.55 296.13 10.77 115.35 394.16 CV (%) 4.5% 6.2% 5.4% 5.7% 7.5% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 194 1525 5484 189 1538 5883 Mean (pg/ml) 194 1525 5484 189 1538 5883 Standard deviation 8.73 94.55 296.13 10.77 115.35 394.16 Standard deviation 8.73 94.55 296.13 10.77 115.35 394.16 CV (%) 4.5% 6.2% 5.4% 5.7% 7.5% 6.7% CV (%) 4.5% 6.2% 5.4% 5.7% 7.5% 6.7%

Sensitivity

<10 pg/mL

Function

Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8 (By similarity) . Possibly involved in helper T-cell function. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

64801 MW

Specificity

Natural and recombinant mouse IL1RL1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-1 receptor-like 1

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the interleukin-1 receptor family.

Protein Name

Interleukin-1 receptor-like 1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.04 0.052 0.093 0.172 0.409 1.028 2.108 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.04 0.052 0.093 0.172 0.409 1.028 2.108 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.04 0.052 0.093 0.172 0.409 1.028 2.108 O.D. 0.033 0.04 0.052 0.093 0.172 0.409 1.028 2.108

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.