Rat TNFSF12/Tweak ELISA Kit PicoKine®

Tumor necrosis factor ligand superfamily member 12 also known as TNF-related weak inducer of apoptosis (TWEAK) is a protein that in humans is encoded by the TNFSF12 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator ofangiogenesis. The TNFSF12 gene lies 878 bp upstream of the putative transcriptional start site of the TNFSF13 gene on chromosome 17p13.1.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 31 31 28 30 1.29 4.3% Sample 2 228 219 215 203 216 8.98 4.1% Sample 3 507 509 522 445 495 29.86 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 31 31 28 30 1.29 4.3% Sample 1 31 31 31 28 30 1.29 4.3% Sample 2 228 219 215 203 216 8.98 4.1% Sample 2 228 219 215 203 216 8.98 4.1% Sample 3 507 509 522 445 495 29.86 6% Sample 3 507 509 522 445 495 29.86 6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 228 507 28 218 506 Standard deviation 1.7 9.8 30.42 1.96 9.59 37.44 CV (%) 5.5% 4.3% 6% 7% 4.4% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 228 507 28 218 506 Standard deviation 1.7 9.8 30.42 1.96 9.59 37.44 CV (%) 5.5% 4.3% 6% 7% 4.4% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 228 507 28 218 506 Mean (pg/ml) 31 228 507 28 218 506 Standard deviation 1.7 9.8 30.42 1.96 9.59 37.44 Standard deviation 1.7 9.8 30.42 1.96 9.59 37.44 CV (%) 5.5% 4.3% 6% 7% 4.4% 7.4% CV (%) 5.5% 4.3% 6% 7% 4.4% 7.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.004 0.073 0.171 0.367 0.759 1.238 1.701 2.057 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.004 0.073 0.171 0.367 0.759 1.238 1.701 2.057 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.004 0.073 0.171 0.367 0.759 1.238 1.701 2.057 O.D. 0.004 0.073 0.171 0.367 0.759 1.238 1.701 2.057

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.