Human FGF19 ELISA Kit PicoKine®

FGF19, Fibroblast growth factor 19, is a protein that in humans is encoded by the FGF19 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. The FGF19 gene is mapped to 11q13.3. The deduced 216-amino acid FGF19 protein contains asignal sequence and 2 cysteine residues that are conserved in the FGF family. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. FGF19 stimulates hepatic protein and glycogen synthesis but does not induce lipogenesis. The effects of FGF19 are independent of the activity of either insulin or the protein kinase Akt and, instead, are mediated through a mitogen-activated protein kinase signaling pathway that activates components of the protein translation machinery and stimulates glycogen synthase activity.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 33 29 34 32 2.27 7% Sample 2 163 189 173 183 177 9.89 5.5% Sample 3 386 396 392 379 388 6.41 1.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 33 29 34 32 2.27 7% Sample 1 35 33 29 34 32 2.27 7% Sample 2 163 189 173 183 177 9.89 5.5% Sample 2 163 189 173 183 177 9.89 5.5% Sample 3 386 396 392 379 388 6.41 1.6% Sample 3 386 396 392 379 388 6.41 1.6%

Cancer, Growth Factors, Growth Factors/Hormones, Neurogenesis, Neurology Process, Neuroscience, Secreted, Signal Transduction, Signaling Pathways, Stem Cells

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 163 386 33 161 403 Standard deviation 2.17 2.212 26.24 2.21 8.37 26.71 CV (%) 6.2% 4.8% 6.8% 6.7% 5.2% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 163 386 33 161 403 Standard deviation 2.17 2.212 26.24 2.21 8.37 26.71 CV (%) 6.2% 4.8% 6.8% 6.7% 5.2% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 163 386 33 161 403 Mean (pg/ml) 35 163 386 33 161 403 Standard deviation 2.17 2.212 26.24 2.21 8.37 26.71 Standard deviation 2.17 2.212 26.24 2.21 8.37 26.71 CV (%) 6.2% 4.8% 6.8% 6.7% 5.2% 7% CV (%) 6.2% 4.8% 6.8% 6.7% 5.2% 7%

Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.036 0.11 0.215 0.442 0.822 1.328 1.876 2.152 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.036 0.11 0.215 0.442 0.822 1.328 1.876 2.152 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.036 0.11 0.215 0.442 0.822 1.328 1.876 2.152 O.D. 0.036 0.11 0.215 0.442 0.822 1.328 1.876 2.152

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.