Mouse CD163 ELISA Kit PicoKine®

Mouse CD163 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Cd163 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD163 (Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich (SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1576 1541 1503 1533 1538 25.99 1.6% Sample 2 5152 5777 5436 5672 5509 240.4 4.3% Sample 3 22633 24816 23376 25733 24139 1209.27 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1576 1541 1503 1533 1538 25.99 1.6% Sample 1 1576 1541 1503 1533 1538 25.99 1.6% Sample 2 5152 5777 5436 5672 5509 240.4 4.3% Sample 2 5152 5777 5436 5672 5509 240.4 4.3% Sample 3 22633 24816 23376 25733 24139 1209.27 5% Sample 3 22633 24816 23376 25733 24139 1209.27 5%

Synonyms

Scavenger receptor cysteine-rich type 1 protein M130; CD163; Soluble CD163; sCD163; Cd163; M130

Gene Name

CD163

Gene ID

93671

UniProt

Q2VLH6

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V39-T1045

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen and mesenteric lymph nodes. .

Applications

ELISA

Field of Research

Atherosclerosis, Cardiovascular, Cell Type Markers, Immunology, Innate Immunity, Macrophage/Inflammation, Vascular Inflammation

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

0.78 ng/mL - 50 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1576 5152 22633 1548 5417 22589 Standard deviation 122.92 221.53 1606.94 131.58 254.59 1784.53 CV (%) 7.8% 4.3% 7.1% 8.5% 4.7% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1576 5152 22633 1548 5417 22589 Standard deviation 122.92 221.53 1606.94 131.58 254.59 1784.53 CV (%) 7.8% 4.3% 7.1% 8.5% 4.7% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1576 5152 22633 1548 5417 22589 Mean (pg/ml) 1576 5152 22633 1548 5417 22589 Standard deviation 122.92 221.53 1606.94 131.58 254.59 1784.53 Standard deviation 122.92 221.53 1606.94 131.58 254.59 1784.53 CV (%) 7.8% 4.3% 7.1% 8.5% 4.7% 7.9% CV (%) 7.8% 4.3% 7.1% 8.5% 4.7% 7.9%

Sensitivity

<20 pg/mL

Function

Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium- dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1 (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

120921 MW

Specificity

Natural and recombinant mouse CD163

Anticoagulant

Heparin

Gene Name Synonym

Scavenger receptor cysteine-rich type 1 protein M130

Subcellular Location

Soluble CD163: Secreted .

Protein Name

Scavenger receptor cysteine-rich type 1 protein M130

Sample Data

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.08 0.126 0.158 0.204 0.312 0.521 1.018 1.894 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.08 0.126 0.158 0.204 0.312 0.521 1.018 1.894 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.08 0.126 0.158 0.204 0.312 0.521 1.018 1.894 O.D. 0.08 0.126 0.158 0.204 0.312 0.521 1.018 1.894

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.