Mouse IL15RA ELISA Kit PicoKine®

Interleukin 15 receptor, alpha subunit is a subunit of the interleukin 15 receptor which is encoded by the gene IL15RA in humans. The human IL15RA gene was mapped to chromosome 10p15-p14 by FISH and the mouse Il15ra gene was mapped to chromosome 2. The cytoplasmic domain of IL15R-alpha, like that of IL2R-alpha, was dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high-affinity binding. At high concentrations, IL15, like IL2, was able to signal through a complex of IL2R-beta and -gamma in the absence of the alpha subunit. The soluble receptor displayed high affinity for IL15 and inhibited both binding of IL15 to the membrane receptor and IL15-induced cell proliferation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 181 196 202 196 193 7.75 4% Sample 2 1121 1352 1324 1168 1241 98.66 7.9% Sample 3 6129 5379 6344 6290 6035 387.19 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 181 196 202 196 193 7.75 4% Sample 1 181 196 202 196 193 7.75 4% Sample 2 1121 1352 1324 1168 1241 98.66 7.9% Sample 2 1121 1352 1324 1168 1241 98.66 7.9% Sample 3 6129 5379 6344 6290 6035 387.19 6.4% Sample 3 6129 5379 6344 6290 6035 387.19 6.4%

Interleukin-15 receptor subunit alpha; IL-15 receptor subunit alpha; IL-15R-alpha; IL-15RA; CD215; Soluble interleukin-15 receptor subunit alpha; sIL-15 receptor subunit alpha; sIL-15R-alpha; sIL-15RA; Il15ra

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 181 1121 6129 165 1015 6536 Standard deviation 12.48 77.34 306.45 11.55 86.9%6 326.8 CV (%) 6.9% 6.9% 5% 7% 8.4% 5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 181 1121 6129 165 1015 6536 Standard deviation 12.48 77.34 306.45 11.55 86.9%6 326.8 CV (%) 6.9% 6.9% 5% 7% 8.4% 5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 181 1121 6129 165 1015 6536 Mean (pg/ml) 181 1121 6129 165 1015 6536 Standard deviation 12.48 77.34 306.45 11.55 86.9%6 326.8 Standard deviation 12.48 77.34 306.45 11.55 86.9%6 326.8 CV (%) 6.9% 6.9% 5% 7% 8.4% 5% CV (%) 6.9% 6.9% 5% 7% 8.4% 5%

High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Signal transduction involves SYK (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.065 0.151 0.231 0.38 0.632 1.035 1.589 2.074 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.065 0.151 0.231 0.38 0.632 1.035 1.589 2.074 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.065 0.151 0.231 0.38 0.632 1.035 1.589 2.074 O.D. 0.065 0.151 0.231 0.38 0.632 1.035 1.589 2.074

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.