Human PON1/Paraoxonase 1 ELISA Kit PicoKine®

Human PON1/Paraoxonase 1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human PON1 in cell culture supernatants, cell lysates, serum and plasma (heparin) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Serum paraoxonase/arylesterase 1 (PON1) also known as aromatic esterase 1 or serum aryldialkylphosphatase 1 is an enzyme that in humans is encoded by the PON1 gene. The PON gene was mapped to chromosome 7q21-q22, and the mouse Pon1 gene was mapped to the proximal end of chromosome 6. PON1 is responsible for hydrolysing organophosphate pesticides and nerve gasses. Polymorphisms in the PON1 gene significantly affect the catalytic ability of the enzyme. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL) .

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 49 52 45 46 48 2.73 5.6% Sample 2 203 223 226 223 218 9.17 4.2% Sample 3 917 923 806 907 888 47.82 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 49 52 45 46 48 2.73 5.6% Sample 1 49 52 45 46 48 2.73 5.6% Sample 2 203 223 226 223 218 9.17 4.2% Sample 2 203 223 226 223 218 9.17 4.2% Sample 3 917 923 806 907 888 47.82 5.3% Sample 3 917 923 806 907 888 47.82 5.3%

Synonyms

Serum paraoxonase/arylesterase 1; PON 1;3.1.1.2;3.1.1.81;3.1.8.1; Aromatic esterase 1; A-esterase 1; K-45; Serum aryldialkylphosphatase 1; PON1; PON

Gene Name

PON1

Gene ID

5444

UniProt

P27169

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A2-L355

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Plasma, associated with HDL (at protein level) . Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas. .

Applications

ELISA

Field of Research

Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Diabetes, Diabetes-associated, Drug Metabolism, Heart Disease, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Oxidative Stress, Pathways and Processes, Redox Metabolism, Signal Transduction

Standard

2 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 49 203 917 47 199 834 Standard deviation 2.15 13.19 42.18 2.16 15.52 40.86 CV (%) 4.4% 6.5% 4.6% 4.6% 7.8% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 49 203 917 47 199 834 Standard deviation 2.15 13.19 42.18 2.16 15.52 40.86 CV (%) 4.4% 6.5% 4.6% 4.6% 7.8% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 49 203 917 47 199 834 Mean (pg/ml) 49 203 917 47 199 834 Standard deviation 2.15 13.19 42.18 2.16 15.52 40.86 Standard deviation 2.15 13.19 42.18 2.16 15.52 40.86 CV (%) 4.4% 6.5% 4.6% 4.6% 7.8% 4.9% CV (%) 4.4% 6.5% 4.6% 4.6% 7.8% 4.9%

Sensitivity

<10 pg/mL

Function

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

39731 MW

Specificity

Natural and recombinant human PON1

Anticoagulant

Heparin

Gene Name Synonym

Serum paraoxonase/arylesterase 1

Subcellular Location

Secreted, extracellular space.

Sequence Similarities

Belongs to the paraoxonase family.

Protein Name

Serum paraoxonase/arylesterase 1

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.041 0.092 0.141 0.23 0.422 0.768 1.348 2.377 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.041 0.092 0.141 0.23 0.422 0.768 1.348 2.377 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.041 0.092 0.141 0.23 0.422 0.768 1.348 2.377 O.D. 0.041 0.092 0.141 0.23 0.422 0.768 1.348 2.377

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.