Human Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine®

Nesfatin-1 is a naturally occurring protein molecule produced by the brains of mammals. It is responsible for regulating appetite and production of body fat. Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the protein precursor, Nucleobindin2 (NUCB2) . Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues. Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets of Langerhans, gastric endocrine cells and adipocytes. Nesfatin-1 plays a role in hunger and energy regulation in an independent manner.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 58 50 49 55 53 3.67 6.9% Sample 2 278 249 272 250 262 12.93 4.9% Sample 3 993 1004 914 846 939 64.06 6.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 58 50 49 55 53 3.67 6.9% Sample 1 58 50 49 55 53 3.67 6.9% Sample 2 278 249 272 250 262 12.93 4.9% Sample 2 278 249 272 250 262 12.93 4.9% Sample 3 993 1004 914 846 939 64.06 6.8% Sample 3 993 1004 914 846 939 64.06 6.8%

Calcium Binding Proteins, Calcium Signaling, Chromatin Binding Proteins, DNA/RNA Binding, Epigenetics and Nuclear Signaling, Signal Transduction, Signaling Pathway

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 58 278 993 62 286 1045 Standard deviation 2.55 17.51 57.20 3.41 20.59 74.19 CV (%) 4.4% 7.2% 5.6% 5.5% 7.2% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 58 278 993 62 286 1045 Standard deviation 2.55 17.51 57.20 3.41 20.59 74.19 CV (%) 4.4% 7.2% 5.6% 5.5% 7.2% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 58 278 993 62 286 1045 Mean (pg/ml) 58 278 993 62 286 1045 Standard deviation 2.55 17.51 57.20 3.41 20.59 74.19 Standard deviation 2.55 17.51 57.20 3.41 20.59 74.19 CV (%) 4.4% 7.2% 5.6% 5.5% 7.2% 7.1% CV (%) 4.4% 7.2% 5.6% 5.5% 7.2% 7.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Golgi apparatus . Membrane ; Peripheral membrane protein . Cytoplasm . Secreted . Endoplasmic reticulum . Nucleus envelope . Golgi retention is mediated by its N-terminal region.

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.084 0.133 0.224 0.365 0.71 1.194 2.105 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.084 0.133 0.224 0.365 0.71 1.194 2.105 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.084 0.133 0.224 0.365 0.71 1.194 2.105 O.D. 0.025 0.084 0.133 0.224 0.365 0.71 1.194 2.105

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.