Human Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine®

Human Nesfatin-1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human NUCB2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Nesfatin-1 is a naturally occurring protein molecule produced by the brains of mammals. It is responsible for regulating appetite and production of body fat. Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the protein precursor, Nucleobindin2 (NUCB2) . Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues. Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets of Langerhans, gastric endocrine cells and adipocytes. Nesfatin-1 plays a role in hunger and energy regulation in an independent manner.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 58 50 49 55 53 3.67 6.9% Sample 2 278 249 272 250 262 12.93 4.9% Sample 3 993 1004 914 846 939 64.06 6.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 58 50 49 55 53 3.67 6.9% Sample 1 58 50 49 55 53 3.67 6.9% Sample 2 278 249 272 250 262 12.93 4.9% Sample 2 278 249 272 250 262 12.93 4.9% Sample 3 993 1004 914 846 939 64.06 6.8% Sample 3 993 1004 914 846 939 64.06 6.8%

Synonyms

Nucleobindin-2; DNA-binding protein NEFA; Gastric cancer antigen Zg4; Prepronesfatin; Nesfatin-1; NUCB2; NEFA

Gene Name

NUCB2

Gene ID

4925

UniProt

P80303

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V25-L106

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Predominantly expressed in spleen, testis and normal stomach. .

Applications

ELISA

Field of Research

Calcium Binding Proteins, Calcium Signaling, Chromatin Binding Proteins, DNA/RNA Binding, Epigenetics and Nuclear Signaling, Signal Transduction, Signaling Pathway

Standard

2 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 58 278 993 62 286 1045 Standard deviation 2.55 17.51 57.20 3.41 20.59 74.19 CV (%) 4.4% 7.2% 5.6% 5.5% 7.2% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 58 278 993 62 286 1045 Standard deviation 2.55 17.51 57.20 3.41 20.59 74.19 CV (%) 4.4% 7.2% 5.6% 5.5% 7.2% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 58 278 993 62 286 1045 Mean (pg/ml) 58 278 993 62 286 1045 Standard deviation 2.55 17.51 57.20 3.41 20.59 74.19 Standard deviation 2.55 17.51 57.20 3.41 20.59 74.19 CV (%) 4.4% 7.2% 5.6% 5.5% 7.2% 7.1% CV (%) 4.4% 7.2% 5.6% 5.5% 7.2% 7.1%

Sensitivity

<10 pg/mL

Function

Calcium-binding protein. May have a role in calcium homeostasis.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

50196 MW

Specificity

Natural and recombinant mouse CCL8

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Nucleobindin-2

Subcellular Location

Golgi apparatus . Membrane ; Peripheral membrane protein . Cytoplasm . Secreted . Endoplasmic reticulum . Nucleus envelope . Golgi retention is mediated by its N-terminal region.

Sequence Similarities

Belongs to the nucleobindin family.

Protein Name

Nucleobindin-2

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.084 0.133 0.224 0.365 0.71 1.194 2.105 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.084 0.133 0.224 0.365 0.71 1.194 2.105 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.084 0.133 0.224 0.365 0.71 1.194 2.105 O.D. 0.025 0.084 0.133 0.224 0.365 0.71 1.194 2.105

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.