Mouse CCL8/MCP-2 ELISA Kit PicoKine®

Mouse CCL8/MCP-2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Ccl8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 8, also known as monocyte chemoattractant protein 2 (MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. Monocyte chemotactic protein 2 (MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 36 34 32 34 1.92 5.6% Sample 2 190 188 192 174 186 7.07 3.8% Sample 3 475 541 471 504 497 28.03 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 36 34 32 34 1.92 5.6% Sample 1 37 36 34 32 34 1.92 5.6% Sample 2 190 188 192 174 186 7.07 3.8% Sample 2 190 188 192 174 186 7.07 3.8% Sample 3 475 541 471 504 497 28.03 5.6% Sample 3 475 541 471 504 497 28.03 5.6%

Synonyms

C-C motif chemokine 8; Monocyte chemoattractant protein 2; Monocyte chemotactic protein 2; MCP-2; Small-inducible cytokine A8; Ccl8; Mcp2, Scya8

Gene Name

CCL8

Gene ID

20307

UniProt

Q9Z121

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: E20-P97

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Chemokines, Immunology, Innate Immunity, Macrophage/Inflammation

Standard

1 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 190 475 33 180 452 Standard deviation 2.66 9.12 28.5 2.73 9 27.12 CV (%) 7.2% 4.8% 6% 8.3% 5% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 190 475 33 180 452 Standard deviation 2.66 9.12 28.5 2.73 9 27.12 CV (%) 7.2% 4.8% 6% 8.3% 5% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 37 190 475 33 180 452 Mean (pg/ml) 37 190 475 33 180 452 Standard deviation 2.66 9.12 28.5 2.73 9 27.12 Standard deviation 2.66 9.12 28.5 2.73 9 27.12 CV (%) 7.2% 4.8% 6% 8.3% 5% 6% CV (%) 7.2% 4.8% 6% 8.3% 5% 6%

Sensitivity

<10 pg/mL

Function

Chemotactic factor that attracts monocytes. This protein can bind heparin (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

11017 MW

Specificity

Natural and recombinant mouse CCL8

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-C motif chemokine 8

Subcellular Location

Secreted .

Protein Name

C-C motif chemokine 8

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.055 0.137 0.182 0.295 0.496 0.897 1.563 2.225 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.055 0.137 0.182 0.295 0.496 0.897 1.563 2.225 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.055 0.137 0.182 0.295 0.496 0.897 1.563 2.225 O.D. 0.055 0.137 0.182 0.295 0.496 0.897 1.563 2.225

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.