Mouse GDF-15 ELISA Kit PicoKine®

GDF-15 (Growth differentiation factor 15), also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by ly interfering with chemokine signaling and integrin activation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 32 31 30 32 2.27 7% Sample 2 233 243 249 254 244 7.82 3.2% Sample 3 386 396 409 421 403 13.2 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 32 31 30 32 2.27 7% Sample 1 36 32 31 30 32 2.27 7% Sample 2 233 243 249 254 244 7.82 3.2% Sample 2 233 243 249 254 244 7.82 3.2% Sample 3 386 396 409 421 403 13.2 3.2% Sample 3 386 396 409 421 403 13.2 3.2%

Cardiovascular, Cell Biology, Cell Cycle, Cell Division, Cytokinesis, Cytoskeleton/ECM, Extracellular Matrix, Growth Factors/Hormones, Hypertrophy, Signal Transduction, Structures

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 233 386 39 238 388 Standard deviation 1.76 10.01 23.16 2.45 14.28 24.3% CV (%) 4.9% 4.3% 6% 6%% 6% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 233 386 39 238 388 Standard deviation 1.76 10.01 23.16 2.45 14.28 24.3% CV (%) 4.9% 4.3% 6% 6%% 6% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 36 233 386 39 238 388 Mean (pg/ml) 36 233 386 39 238 388 Standard deviation 1.76 10.01 23.16 2.45 14.28 24.3% Standard deviation 1.76 10.01 23.16 2.45 14.28 24.3% CV (%) 4.9% 4.3% 6% 6%% 6% 6.6% CV (%) 4.9% 4.3% 6% 6%% 6% 6.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.041 0.092 0.14 0.252 0.396 0.707 1.105 1.849 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.041 0.092 0.14 0.252 0.396 0.707 1.105 1.849 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.041 0.092 0.14 0.252 0.396 0.707 1.105 1.849 O.D. 0.041 0.092 0.14 0.252 0.396 0.707 1.105 1.849

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.