Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine®

Mouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Tlr2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Toll-like receptor 2 also known as TLR-2 is a protein that in humans is encoded by the TLR2 gene. TLR2 has also been designated as CD282 (cluster of differentiation 282) . TLR-2 plays a role in the immune system. TLR-2 is a membrane protein, a receptor, which is expressed on the surface of certain cells and recognizes foreign substances and passes on appropriate signals to the cells of the immune system. TLR2 expresses as 4- and 4.4-kb mRNAs in heart, brain, and muscle.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 304 323 310 307 311 7.24 2.3% Sample 2 1448 1379 1487 1331 1411 60.35 4.2% Sample 3 4661 5150 4522 4797 4782 233.39 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 304 323 310 307 311 7.24 2.3% Sample 1 304 323 310 307 311 7.24 2.3% Sample 2 1448 1379 1487 1331 1411 60.35 4.2% Sample 2 1448 1379 1487 1331 1411 60.35 4.2% Sample 3 4661 5150 4522 4797 4782 233.39 4.8% Sample 3 4661 5150 4522 4797 4782 233.39 4.8%

Synonyms

Toll-like receptor 2; CD282; Tlr2

Gene Name

TLR2

Gene ID

24088

UniProt

Q9QUN7

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q25-L590

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Detected in a macrophage cell line, smooth muscle, lung, spleen, thymus, brain and adipose tissue.

Applications

ELISA

Field of Research

Adapters, Atherosclerosis, Cardiovascular, Defensin, Human Protein, Immunoglobulins, Immunology, Innate Immunity, Macrophage/Inflammation, Microbiology, Protein, Receptors, Signal Transduction, TLR Signaling, Transmembrane, Vascular Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 304 1448 4661 301 1423 4925 Standard deviation 21.58 65.76 195.76 26.48 91.07 280.72 CV (%) 7.1% 4.5% 4.2% 8.8% 6.4% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 304 1448 4661 301 1423 4925 Standard deviation 21.58 65.76 195.76 26.48 91.07 280.72 CV (%) 7.1% 4.5% 4.2% 8.8% 6.4% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 304 1448 4661 301 1423 4925 Mean (pg/ml) 304 1448 4661 301 1423 4925 Standard deviation 21.58 65.76 195.76 26.48 91.07 280.72 Standard deviation 21.58 65.76 195.76 26.48 91.07 280.72 CV (%) 7.1% 4.5% 4.2% 8.8% 6.4% 5.7% CV (%) 7.1% 4.5% 4.2% 8.8% 6.4% 5.7%

Sensitivity

<10 pg/mL

Function

Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

89449 MW

Specificity

Natural and recombinant mouse TLR2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Toll-like receptor 2

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, phagosome membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the Toll-like receptor family.

Protein Name

Toll-like receptor 2

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.079 0.127 0.223 0.389 0.734 1.254 1.891 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.079 0.127 0.223 0.389 0.734 1.254 1.891 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.079 0.127 0.223 0.389 0.734 1.254 1.891 O.D. 0.015 0.079 0.127 0.223 0.389 0.734 1.254 1.891

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.