Human TLR1/TIL ELISA Kit PicoKine®

Human TLR1 / TIL ELISA Kit PicoKine® (96 Tests) . Quantitate Human TLR1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

TLR1 is a member of the Toll-like receptor family (TLR) of pattern recognition receptors of the innate immune system. TIR1 gene was mapped to 4p14 by fluorescence in situ hybridization. TLR1 recognizes pathogen-associated molecular pattern with a specificity for gram-positive bacteria. TLR1 has also been designated as CD281 (cluster of differentiation 281) . TLR1 interacts with TLR2 to recognize the lipid configuration of the native mycobacterial lipoprotein as well as several triacylated lipopeptides.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 204 208 203 192 201 5.93 2.9% Sample 2 1142 1045 1032 1050 1067 43.65 4% Sample 3 6099 6066 5527 6006 5924 231.9 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 204 208 203 192 201 5.93 2.9% Sample 1 204 208 203 192 201 5.93 2.9% Sample 2 1142 1045 1032 1050 1067 43.65 4% Sample 2 1142 1045 1032 1050 1067 43.65 4% Sample 3 6099 6066 5527 6006 5924 231.9 3.9% Sample 3 6099 6066 5527 6006 5924 231.9 3.9%

Synonyms

Toll-like receptor 1; Toll/interleukin-1 receptor-like protein; TIL; CD281; TLR1; KIAA0012

Gene Name

TLR1

Gene ID

7096

UniProt

Q15399

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S22-N578

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine.

Applications

ELISA

Field of Research

Adapters, Immunoglobulins, Immunology, Innate Immunity, Macrophage/Inflammation, NFKB Pathway, Nuclear Signaling, Receptors, Signal Transduction, Signaling Pathway, TLR Signaling, Transmembrane

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 204 1142 6099 218 1081 6218 Standard deviation 11.22 78.79 378.13 16.2%1 77.83 472.56 CV (%) 5.5% 6.9% 6.2% 7.3% 7.2% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 204 1142 6099 218 1081 6218 Standard deviation 11.22 78.79 378.13 16.2%1 77.83 472.56 CV (%) 5.5% 6.9% 6.2% 7.3% 7.2% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 204 1142 6099 218 1081 6218 Mean (pg/ml) 204 1142 6099 218 1081 6218 Standard deviation 11.22 78.79 378.13 16.2%1 77.83 472.56 Standard deviation 11.22 78.79 378.13 16.2%1 77.83 472.56 CV (%) 5.5% 6.9% 6.2% 7.3% 7.2% 7.6% CV (%) 5.5% 6.9% 6.2% 7.3% 7.2% 7.6%

Sensitivity

<10 pg/mL

Function

Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

90291 MW

Specificity

Natural and recombinant human TLR1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Toll-like receptor 1

Subcellular Location

Cell membrane ; Single-pass type I membrane protein . Cytoplasmic vesicle, phagosome membrane ; Single-pass type I membrane protein .

Sequence Similarities

Belongs to the Toll-like receptor family.

Protein Name

Toll-like receptor 1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.088 0.151 0.258 0.458 0.817 1.395 2.004 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.088 0.151 0.258 0.458 0.817 1.395 2.004 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.088 0.151 0.258 0.458 0.817 1.395 2.004 O.D. 0.017 0.088 0.151 0.258 0.458 0.817 1.395 2.004

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.