Mouse CCL6/C10 ELISA Kit PicoKine®

Mouse CCL6/C10 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Ccl6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 6 (CCL6) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents.In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin (e.g. the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6 is greatly reduced. CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 33 30 31 31 1.29 4.1% Sample 2 164 163 160 168 163 2.86 1.7% Sample 3 462 418 416 460 439 22.02 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 33 30 31 31 1.29 4.1% Sample 1 33 33 30 31 31 1.29 4.1% Sample 2 164 163 160 168 163 2.86 1.7% Sample 2 164 163 160 168 163 2.86 1.7% Sample 3 462 418 416 460 439 22.02 5% Sample 3 462 418 416 460 439 22.02 5%

Synonyms

C-C motif chemokine 6; Protein C10; Small-inducible cytokine A6; CCL6 (22-95) ; CCL6 (23-95) ; Ccl6; C10, Scya6

Gene Name

Ccl6

Gene ID

20305

UniProt

P27784

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G22-A116

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in myelopoietic bone marrow cultures stimulated by GM-CSF.

Applications

ELISA

Field of Research

Chemokines, Immunology, Innate Immunity

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 164 462 34 165 473 Standard deviation 2.04 11.48 26.79 2.14 13.36 33.58 CV (%) 6.2% 7% 5.8% 8.1%% 8.1% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 164 462 34 165 473 Standard deviation 2.04 11.48 26.79 2.14 13.36 33.58 CV (%) 6.2% 7% 5.8% 8.1%% 8.1% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 164 462 34 165 473 Mean (pg/ml) 33 164 462 34 165 473 Standard deviation 2.04 11.48 26.79 2.14 13.36 33.58 Standard deviation 2.04 11.48 26.79 2.14 13.36 33.58 CV (%) 6.2% 7% 5.8% 8.1%% 8.1% 7.1% CV (%) 6.2% 7% 5.8% 8.1%% 8.1% 7.1%

Sensitivity

<10 pg/mL

Function

CCL6 (22-95) and CCL6 (23-95) are potent chemoattractants. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

12984 MW

Specificity

Natural and recombinant mouse CCL6

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-C motif chemokine 6

Subcellular Location

Secreted.

Protein Name

C-C motif chemokine 6

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.096 0.22 0.286 0.505 0.814 1.279 1.97 2.589 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.096 0.22 0.286 0.505 0.814 1.279 1.97 2.589 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.096 0.22 0.286 0.505 0.814 1.279 1.97 2.589 O.D. 0.096 0.22 0.286 0.505 0.814 1.279 1.97 2.589

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.