Human CCL14/HCC-1 ELISA Kit PicoKine®

Chemokine (C-C motif) ligand 14 (CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. In addition, HCC-1 enhanced the proliferation of CD34+ myeloid progenitor cells. It was as effective as MIP-1 alpha, but about 100-fold less potent. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 34 36 35 35 1.11 3.1% Sample 2 238 250 251 216 238 14.09 5.9% Sample 3 445 501 523 499 492 28.72 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 34 36 35 35 1.11 3.1% Sample 1 37 34 36 35 35 1.11 3.1% Sample 2 238 250 251 216 238 14.09 5.9% Sample 2 238 250 251 216 238 14.09 5.9% Sample 3 445 501 523 499 492 28.72 5.8% Sample 3 445 501 523 499 492 28.72 5.8%

C-C motif chemokine 14; Chemokine CC-1/CC-3; HCC-1/HCC-3; HCC-1 (1-74) ; NCC-2; Small-inducible cytokine A14; HCC-1 (3-74) ; HCC-1 (4-74) ; HCC-1 (9-74) ; CCL14; NCC2, SCYA14

Expressed constitutively in several normal tissues: spleen, liver, skeletal and heart muscle, gut, and bone marrow, present at high concentrations (1-80 nM) in plasma. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 238 445 40 227 446 Standard deviation 1.92 13.8 25.81 2.08 15.89 33.45 CV (%) 5.2% 5.8% 5.8% 5.2% 7% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 238 445 40 227 446 Standard deviation 1.92 13.8 25.81 2.08 15.89 33.45 CV (%) 5.2% 5.8% 5.8% 5.2% 7% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 37 238 445 40 227 446 Mean (pg/ml) 37 238 445 40 227 446 Standard deviation 1.92 13.8 25.81 2.08 15.89 33.45 Standard deviation 1.92 13.8 25.81 2.08 15.89 33.45 CV (%) 5.2% 5.8% 5.8% 5.2% 7% 7.5% CV (%) 5.2% 5.8% 5.8% 5.2% 7% 7.5%

Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca (2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1 (9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.122 0.202 0.376 0.698 1.158 1.824 2.372 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.122 0.202 0.376 0.698 1.158 1.824 2.372 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.122 0.202 0.376 0.698 1.158 1.824 2.372 O.D. 0.023 0.122 0.202 0.376 0.698 1.158 1.824 2.372

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.