Mouse TNFRSF13C/BAFFR ELISA Kit PicoKine®

Mouse TNFRSF13C/BAFFR ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Tnfrsf13c in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Tumor necrosis factor receptor superfamily member 13C also known as B-cell-activating factor receptor or BAFF receptor is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 112 109 105 109 2.54 2.3% Sample 2 605 673 577 616 617 34.92 5.6% Sample 3 2620 2839 2527 2525 2627 127.86 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 112 109 105 109 2.54 2.3% Sample 1 110 112 109 105 109 2.54 2.3% Sample 2 605 673 577 616 617 34.92 5.6% Sample 2 605 673 577 616 617 34.92 5.6% Sample 3 2620 2839 2527 2525 2627 127.86 4.8% Sample 3 2620 2839 2527 2525 2627 127.86 4.8%

Synonyms

Tumor necrosis factor receptor superfamily member 13C; B-cell maturation defect; B-cell-activating factor receptor; BAFF receptor; BAFF-R; BLyS receptor 3; CD268; Tnfrsf13c; Baffr, Bcmd, Br3

Gene Name

TNFRSF13C

Gene ID

72049

UniProt

Q9D8D0

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S10-A71

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Highly expressed in spleen and testis; detected at lower levels in lung and thymus.

Applications

ELISA

Field of Research

Growth Factors/Hormones, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

78 pg/mL - 5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 605 2620 117 626 2583 Standard deviation 4.4 47.19 117.9 7.8%1 60.72 154.98 CV (%) 4% 7.8% 4.5% 4.8% 9.7% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 605 2620 117 626 2583 Standard deviation 4.4 47.19 117.9 7.8%1 60.72 154.98 CV (%) 4% 7.8% 4.5% 4.8% 9.7% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 110 605 2620 117 626 2583 Mean (pg/ml) 110 605 2620 117 626 2583 Standard deviation 4.4 47.19 117.9 7.8%1 60.72 154.98 Standard deviation 4.4 47.19 117.9 7.8%1 60.72 154.98 CV (%) 4% 7.8% 4.5% 4.8% 9.7% 6% CV (%) 4% 7.8% 4.5% 4.8% 9.7% 6%

Sensitivity

<10 pg/mL

Function

B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

18798 MW

Specificity

Natural and recombinant mouse TNFRSF13C

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Tumor necrosis factor receptor superfamily member 13C

Subcellular Location

Membrane ; Single-pass type III membrane protein .

Protein Name

Tumor necrosis factor receptor superfamily member 13C

Sample Data

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.007 0.07 0.128 0.24 0.471 0.828 1.465 2.066 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.007 0.07 0.128 0.24 0.471 0.828 1.465 2.066 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.007 0.07 0.128 0.24 0.471 0.828 1.465 2.066 O.D. 0.007 0.07 0.128 0.24 0.471 0.828 1.465 2.066

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.