Human TNFRSF13C/BAFFR ELISA Kit PicoKine®

Tumor necrosis factor receptor superfamily member 13C also known as B-cell-activating factor receptor or BAFF receptor is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 147 162 135 138 145 10.5 7.2% Sample 2 683 747 709 652 697 34.86 5% Sample 3 2750 3116 2752 2808 2856 151.62 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 147 162 135 138 145 10.5 7.2% Sample 1 147 162 135 138 145 10.5 7.2% Sample 2 683 747 709 652 697 34.86 5% Sample 2 683 747 709 652 697 34.86 5% Sample 3 2750 3116 2752 2808 2856 151.62 5.3% Sample 3 2750 3116 2752 2808 2856 151.62 5.3%

Tumor necrosis factor receptor superfamily member 13C; B-cell-activating factor receptor; BAFF receptor; BAFF-R; BLyS receptor 3; CD268; TNFRSF13C; BAFFR, BR3

Highly expressed in spleen and lymph node, and in resting B-cells. Detected at lower levels in activated B-cells, resting CD4+ T-cells, in thymus and peripheral blood leukocytes.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 147 683 2750 156 700 2531 Standard deviation 10.29 49.85 206.25 13.88 62.3 227.05 CV (%) 7% 7.3% 7.5% 8.9% 8.9% 8.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 147 683 2750 156 700 2531 Standard deviation 10.29 49.85 206.25 13.88 62.3 227.05 CV (%) 7% 7.3% 7.5% 8.9% 8.9% 8.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 147 683 2750 156 700 2531 Mean (pg/ml) 147 683 2750 156 700 2531 Standard deviation 10.29 49.85 206.25 13.88 62.3 227.05 Standard deviation 10.29 49.85 206.25 13.88 62.3 227.05 CV (%) 7% 7.3% 7.5% 8.9% 8.9% 8.9% CV (%) 7% 7.3% 7.5% 8.9% 8.9% 8.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.031 0.12 0.192 0.341 0.604 1.071 1.875 2.426 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.031 0.12 0.192 0.341 0.604 1.071 1.875 2.426 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.031 0.12 0.192 0.341 0.604 1.071 1.875 2.426 O.D. 0.031 0.12 0.192 0.341 0.604 1.071 1.875 2.426

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.