Human CCL15/Mip 1 Delta ELISA Kit PicoKine®

Chemokine (C-C motif) ligand 15 (CCL15) is a small cytokine belonging to the CC chemokine family that is also known as leukotactin-1, MIP5 and HCC-2. CCL15 is expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3. The human CCL15 gene spans four exons and is located in a head-to-tail orientation on chromosome 17 with the gene of another CC chemokine known as CCL14.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 29 29 33 31 2 6.4% Sample 2 185 185 181 184 183 1.63 0.8% Sample 3 439 430 478 439 446 18.55 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 33 29 29 33 31 2 6.4% Sample 1 33 29 29 33 31 2 6.4% Sample 2 185 185 181 184 183 1.63 0.8% Sample 2 185 185 181 184 183 1.63 0.8% Sample 3 439 430 478 439 446 18.55 4.1% Sample 3 439 430 478 439 446 18.55 4.1%

C-C motif chemokine 15; Chemokine CC-2; HCC-2; Leukotactin-1; LKN-1; MIP-1 delta; Macrophage inflammatory protein 5; MIP-5; Mrp-2b; NCC-3; Small-inducible cytokine A15; CCL15 (22-92) ; CCL15 (25-92) ; CCL15 (29-92) ; CCL15; MIP5, NCC3, SCYA15

Most abundant in heart, skeletal muscle and adrenal gland. Lower levels in placenta, liver, pancreas and bone marrow. CCL15 (22-92), CCL15 (25-92) and CCL15 (29-92) are found in high levels in synovial fluids from rheumatoid patients. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 185 439 31 188 417 Standard deviation 2.31 9.8 31.6 2.75 13.34 37.11 CV (%) 7% 5.3% 7.2% 8.9% 7.1% 8.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 33 185 439 31 188 417 Standard deviation 2.31 9.8 31.6 2.75 13.34 37.11 CV (%) 7% 5.3% 7.2% 8.9% 7.1% 8.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 33 185 439 31 188 417 Mean (pg/ml) 33 185 439 31 188 417 Standard deviation 2.31 9.8 31.6 2.75 13.34 37.11 Standard deviation 2.31 9.8 31.6 2.75 13.34 37.11 CV (%) 7% 5.3% 7.2% 8.9% 7.1% 8.9% CV (%) 7% 5.3% 7.2% 8.9% 7.1% 8.9%

Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15 (22-92), CCL15 (25-92) and CCL15 (29-92) are more potent chemoattractants than the small-inducible cytokine A15. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.027 0.093 0.129 0.224 0.421 0.725 1.326 2.003 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.027 0.093 0.129 0.224 0.421 0.725 1.326 2.003 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.027 0.093 0.129 0.224 0.421 0.725 1.326 2.003 O.D. 0.027 0.093 0.129 0.224 0.421 0.725 1.326 2.003

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.