Human Elafin/PI3 ELISA Kit PicoKine®

Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase (SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13.12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 64 53 57 58 58 3.93 6.7% Sample 2 263 250 260 236 252 10.54 4.1% Sample 3 893 966 957 1042 964 52.86 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 64 53 57 58 58 3.93 6.7% Sample 1 64 53 57 58 58 3.93 6.7% Sample 2 263 250 260 236 252 10.54 4.1% Sample 2 263 250 260 236 252 10.54 4.1% Sample 3 893 966 957 1042 964 52.86 5.4% Sample 3 893 966 957 1042 964 52.86 5.4%

Elafin; Elastase-specific inhibitor; ESI; Peptidase inhibitor 3; PI-3; Protease inhibitor WAP3; Skin-derived antileukoproteinase; SKALP; WAP four-disulfide core domain protein 14; PI3; WAP3, WFDC14

Cell Biology, Cell Cycle, Immunology, Innate Immunity, Kinases/Phosphatases, Lipid Signaling, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction, Signaling Pathway, TLR Signaling

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 64 263 893 70 273 891 Standard deviation 4.22 12.36 51.79 4.9 15.56 61.47 CV (%) 6.6% 4.7% 5.8% 7% 5.7% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 64 263 893 70 273 891 Standard deviation 4.22 12.36 51.79 4.9 15.56 61.47 CV (%) 6.6% 4.7% 5.8% 7% 5.7% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 64 263 893 70 273 891 Mean (pg/ml) 64 263 893 70 273 891 Standard deviation 4.22 12.36 51.79 4.9 15.56 61.47 Standard deviation 4.22 12.36 51.79 4.9 15.56 61.47 CV (%) 6.6% 4.7% 5.8% 7% 5.7% 6.9% CV (%) 6.6% 4.7% 5.8% 7% 5.7% 6.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.049 0.198 0.306 0.454 0.679 1.063 1.678 2.486 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.049 0.198 0.306 0.454 0.679 1.063 1.678 2.486 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.049 0.198 0.306 0.454 0.679 1.063 1.678 2.486 O.D. 0.049 0.198 0.306 0.454 0.679 1.063 1.678 2.486

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.