Human Granzyme B ELISA Kit PicoKine®

Granzyme B is a serine protease that in humans is encoded by the GZMB gene. Granzyme B is expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. CTL and NK cells share the remarkable ability to recognize specific infected target cells. They are thought to protect their host by inducing apoptosis of cells that bear on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 36 36 35 35 0.5 1.4% Sample 2 170 190 185 175 180 7.9 4.3% Sample 3 476 469 464 420 457 21.92 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 36 36 35 35 0.5 1.4% Sample 1 35 36 36 35 35 0.5 1.4% Sample 2 170 190 185 175 180 7.9 4.3% Sample 2 170 190 185 175 180 7.9 4.3% Sample 3 476 469 464 420 457 21.92 4.7% Sample 3 476 469 464 420 457 21.92 4.7%

Granzyme B;3.4.21.79; C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E; GZMB; CGL1, CSPB, CTLA1, GRB

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 170 476 38 176 487 Standard deviation 2.73 7.14 27.6 3.26 10.2 32.62 CV (%) 7.8% 4.2% 5.8% 8.6% 5.8% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 170 476 38 176 487 Standard deviation 2.73 7.14 27.6 3.26 10.2 32.62 CV (%) 7.8% 4.2% 5.8% 8.6% 5.8% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 170 476 38 176 487 Mean (pg/ml) 35 170 476 38 176 487 Standard deviation 2.73 7.14 27.6 3.26 10.2 32.62 Standard deviation 2.73 7.14 27.6 3.26 10.2 32.62 CV (%) 7.8% 4.2% 5.8% 8.6% 5.8% 6.7% CV (%) 7.8% 4.2% 5.8% 8.6% 5.8% 6.7%

This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.026 0.056 0.074 0.115 0.198 0.439 0.954 1.971 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.026 0.056 0.074 0.115 0.198 0.439 0.954 1.971 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.026 0.056 0.074 0.115 0.198 0.439 0.954 1.971 O.D. 0.026 0.056 0.074 0.115 0.198 0.439 0.954 1.971

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.