Human Galectin-9 / Gal 9 ELISA Kit PicoKine®

Galectin-9, also called HUAT or LGALS9A is a protein that in humans is encoded by the LGALS9 gene. This gene is mapped to 17q11.2. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. The protein has N- and C- terminal carbohydrate-binding domains connected by a link peptide.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 168 165 163 179 168 6.17 3.6% Sample 2 731 656 726 619 683 47.37 6.9% Sample 3 3580 3263 3243 3664 3437 187 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 168 165 163 179 168 6.17 3.6% Sample 1 168 165 163 179 168 6.17 3.6% Sample 2 731 656 726 619 683 47.37 6.9% Sample 2 731 656 726 619 683 47.37 6.9% Sample 3 3580 3263 3243 3664 3437 187 5.4% Sample 3 3580 3263 3243 3664 3437 187 5.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 168 731 3580 177 792 3920 Standard deviation 7.90 51.9 218.38 9.55 66.52 274.4 CV (%) 4.7% 7.1% 6.1% 5.4% 8.4% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 168 731 3580 177 792 3920 Standard deviation 7.90 51.9 218.38 9.55 66.52 274.4 CV (%) 4.7% 7.1% 6.1% 5.4% 8.4% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 168 731 3580 177 792 3920 Mean (pg/ml) 168 731 3580 177 792 3920 Standard deviation 7.90 51.9 218.38 9.55 66.52 274.4 Standard deviation 7.90 51.9 218.38 9.55 66.52 274.4 CV (%) 4.7% 7.1% 6.1% 5.4% 8.4% 7% CV (%) 4.7% 7.1% 6.1% 5.4% 8.4% 7%

Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.039 0.074 0.113 0.198 0.371 0.675 1.352 2.023 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.039 0.074 0.113 0.198 0.371 0.675 1.352 2.023 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.039 0.074 0.113 0.198 0.371 0.675 1.352 2.023 O.D. 0.039 0.074 0.113 0.198 0.371 0.675 1.352 2.023

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.