Rat Cystatin C/CST3 ELISA Kit PicoKine®

Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure. Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In mouses, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes) . Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 824 793 785 735 784 31.94 4% Sample 2 2517 2693 2642 2687 2634 70.78 2.6% Sample 3 8104 8014 8243 8197 8139 88.07 1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 824 793 785 735 784 31.94 4% Sample 1 824 793 785 735 784 31.94 4% Sample 2 2517 2693 2642 2687 2634 70.78 2.6% Sample 2 2517 2693 2642 2687 2634 70.78 2.6% Sample 3 8104 8014 8243 8197 8139 88.07 1% Sample 3 8104 8014 8243 8197 8139 88.07 1%

Apoptosis, Atherosclerosis, Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Intracellular, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 824 2517 8104 756 2556 7350 Standard deviation 34.6 128.36 445.72 39.31 146.49 470.40 CV (%) 4.2% 6.4% 5.5% 5.2% 5.7% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 824 2517 8104 756 2556 7350 Standard deviation 34.6 128.36 445.72 39.31 146.49 470.40 CV (%) 4.2% 6.4% 5.5% 5.2% 5.7% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 824 2517 8104 756 2556 7350 Mean (pg/ml) 824 2517 8104 756 2556 7350 Standard deviation 34.6 128.36 445.72 39.31 146.49 470.40 Standard deviation 34.6 128.36 445.72 39.31 146.49 470.40 CV (%) 4.2% 6.4% 5.5% 5.2% 5.7% 6.4% CV (%) 4.2% 6.4% 5.5% 5.2% 5.7% 6.4%

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.011 0.074 0.143 0.269 0.516 0.917 1.563 2.295 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.011 0.074 0.143 0.269 0.516 0.917 1.563 2.295 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.011 0.074 0.143 0.269 0.516 0.917 1.563 2.295 O.D. 0.011 0.074 0.143 0.269 0.516 0.917 1.563 2.295

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.