Rat TGF-Beta 3 ELISA Kit PicoKine®

Transforming growth factor beta 3 (TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin (CTNNB1) via nuclear phospho-Smad2 and Smad4.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 77 75 69 73 73 2.95 4% Sample 2 234 208 238 199 219 16.61 7.5% Sample 3 841 937 936 884 899 40 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 77 75 69 73 73 2.95 4% Sample 1 77 75 69 73 73 2.95 4% Sample 2 234 208 238 199 219 16.61 7.5% Sample 2 234 208 238 199 219 16.61 7.5% Sample 3 841 937 936 884 899 40 4.4% Sample 3 841 937 936 884 899 40 4.4%

Angiogenesis, Cancer, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, TGF Beta

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 77 234 841 81 242 805 Standard deviation 5.22 14.97 42.89 7.12 18.15 41.86 CV (%) 7.7% 6.4% 5.2% 8.8% 7.5% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 77 234 841 81 242 805 Standard deviation 5.22 14.97 42.89 7.12 18.15 41.86 CV (%) 7.7% 6.4% 5.2% 8.8% 7.5% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 77 234 841 81 242 805 Mean (pg/ml) 77 234 841 81 242 805 Standard deviation 5.22 14.97 42.89 7.12 18.15 41.86 Standard deviation 5.22 14.97 42.89 7.12 18.15 41.86 CV (%) 7.7% 6.4% 5.2% 8.8% 7.5% 5.2% CV (%) 7.7% 6.4% 5.2% 8.8% 7.5% 5.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.027 0.203 0.314 0.497 0.773 1.205 1.861 2.763 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.027 0.203 0.314 0.497 0.773 1.205 1.861 2.763 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.027 0.203 0.314 0.497 0.773 1.205 1.861 2.763 O.D. 0.027 0.203 0.314 0.497 0.773 1.205 1.861 2.763

Activate the sample Cell culture supernatants, urine: add activating reagent pro rata, i.e. add 20μl of Solution A into 100μl of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6. Serum, plasma (EDTA) : add activating reagent pro rata, i.e. add 20μl of Solution A into 40μl of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6. It is unnecessary to activate the recombinant TGF-beta 3. Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration. Activate the sample Cell culture supernatants, urine: add activating reagent pro rata, i.e. add 20μl of Solution A into 100μl of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6. Serum, plasma (EDTA) : add activating reagent pro rata, i.e. add 20μl of Solution A into 40μl of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6. It is unnecessary to activate the recombinant TGF-beta 3. Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.