Mouse IL-31/Interleukin-31 ELISA Kit PicoKine®

Interleukin-31, also known as IL-31 is a protein that in humans is encoded by the IL31 gene. The IL31 gene is mapped to chromosome 12q24.31 and the mouse gene maps to a syntenic region on chromosome 5. IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA and OSMR that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 209 213 189 176 196 15.03 7.6% Sample 2 790 810 714 798 778 37.62 4.8% Sample 3 1270 1290 1315 1320 1298 20.11 1.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 209 213 189 176 196 15.03 7.6% Sample 1 209 213 189 176 196 15.03 7.6% Sample 2 790 810 714 798 778 37.62 4.8% Sample 2 790 810 714 798 778 37.62 4.8% Sample 3 1270 1290 1315 1320 1298 20.11 1.5% Sample 3 1270 1290 1315 1320 1298 20.11 1.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 209 790 1270 212 719 1315 Standard deviation 9.82 60.83 55.88 11.66 58.95 68.38 CV (%) 4.7% 7.7% 4.4% 5.5% 8.2% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 209 790 1270 212 719 1315 Standard deviation 9.82 60.83 55.88 11.66 58.95 68.38 CV (%) 4.7% 7.7% 4.4% 5.5% 8.2% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 209 790 1270 212 719 1315 Mean (pg/ml) 209 790 1270 212 719 1315 Standard deviation 9.82 60.83 55.88 11.66 58.95 68.38 Standard deviation 9.82 60.83 55.88 11.66 58.95 68.38 CV (%) 4.7% 7.7% 4.4% 5.5% 8.2% 5.2% CV (%) 4.7% 7.7% 4.4% 5.5% 8.2% 5.2%

Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.0 0.102 0.193 0.409 0.788 1.312 1.871 1.996 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.0 0.102 0.193 0.409 0.788 1.312 1.871 1.996 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.0 0.102 0.193 0.409 0.788 1.312 1.871 1.996 O.D. 0.0 0.102 0.193 0.409 0.788 1.312 1.871 1.996

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.