Human ICAM-2 ELISA Kit PicoKine®

Human ICAM-2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human ICAM2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Intercellular adhesion molecule 2 (ICAM2), also known as CD102 (Cluster of Differentiation 102) is an integral membrane protein that has 2 immunoglobulin-like domains. This gene is mapped to 17q23.3. The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 640 642 715 684 670 31.24 4.6% Sample 2 2403 2237 2185 2586 2352 156.89 6.6% Sample 3 7918 7803 8915 7900 8134 453.02 5.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 640 642 715 684 670 31.24 4.6% Sample 1 640 642 715 684 670 31.24 4.6% Sample 2 2403 2237 2185 2586 2352 156.89 6.6% Sample 2 2403 2237 2185 2586 2352 156.89 6.6% Sample 3 7918 7803 8915 7900 8134 453.02 5.5% Sample 3 7918 7803 8915 7900 8134 453.02 5.5%

Synonyms

Intercellular adhesion molecule 2; ICAM-2; CD102; ICAM2

Gene Name

ICAM2

Gene ID

3384

UniProt

P13598

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: K25-Q223

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cell Type Markers, Hematopoietic Progenitors, Immunology, Lymphoid, Stem Cells, T Lymphocytic Lineage

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 640 2403 7918 592 2447 7410 Standard deviation 38.4 151.38 443.4 38.48 196.5% 437.19 CV (%) 6% 8.1%% 5.6% 6.5% 8.1% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 640 2403 7918 592 2447 7410 Standard deviation 38.4 151.38 443.4 38.48 196.5% 437.19 CV (%) 6% 8.1%% 5.6% 6.5% 8.1% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 640 2403 7918 592 2447 7410 Mean (pg/ml) 640 2403 7918 592 2447 7410 Standard deviation 38.4 151.38 443.4 38.48 196.5% 437.19 Standard deviation 38.4 151.38 443.4 38.48 196.5% 437.19 CV (%) 6% 8.1%% 5.6% 6.5% 8.1% 5.9% CV (%) 6% 8.1%% 5.6% 6.5% 8.1% 5.9%

Sensitivity

<10 pg/mL

Function

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) . ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen- specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

30654 MW

Specificity

Natural and recombinant human ICAM-2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Intercellular adhesion molecule 2

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the immunoglobulin superfamily. ICAM family.

Protein Name

Intercellular adhesion molecule 2

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.009 0.069 0.128 0.227 0.403 0.706 1.285 2.001 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.009 0.069 0.128 0.227 0.403 0.706 1.285 2.001 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.009 0.069 0.128 0.227 0.403 0.706 1.285 2.001 O.D. 0.009 0.069 0.128 0.227 0.403 0.706 1.285 2.001

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.