Mouse TNFRSF4/OX40 ELISA Kit PicoKine®

Tumor necrosis factor receptor superfamily, member4, also known as ACT35 or CD134 is a cell surface glycoprotein that was discovered through the production of a monoclonal antibody raised against the HUT-102 cell line. It belongs to the tumor necrosis factor receptor superfamily. CD134 was mapped to 1p36 by fluorescence in situ hybridization. CD134 is the primary receptor for feline immunodeficiency virus. CD134 expression can promote viral binding and renders cells permissive for viral entry, productive infection, and syncytium formation. Stimulating the receptor can improve the response to a powerful virus vector and may be useful in vaccine development.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 92 86 86 91 5.74 6.3% Sample 2 233 215 231 255 233 14.23 6.1% Sample 3 894 988 987 983 963 39.88 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 92 86 86 91 5.74 6.3% Sample 1 100 92 86 86 91 5.74 6.3% Sample 2 233 215 231 255 233 14.23 6.1% Sample 2 233 215 231 255 233 14.23 6.1% Sample 3 894 988 987 983 963 39.88 4.1% Sample 3 894 988 987 983 963 39.88 4.1%

Adaptive Immunity, Autoimmune, B Cells, Cytokines, Hematopoietic Progenitors, Immune System Diseases, Immunology, Innate Immunity, Lymphoid, Stem Cells, T Cells, T Lymphocytic Lineage, TNF Superfamily

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 233 894 97 255 891 Standard deviation 5.8 14.91 39.33 5.72 16.42 43.65 CV (%) 5.8% 6.4% 4.4% 5.9% 6.4% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 233 894 97 255 891 Standard deviation 5.8 14.91 39.33 5.72 16.42 43.65 CV (%) 5.8% 6.4% 4.4% 5.9% 6.4% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 100 233 894 97 255 891 Mean (pg/ml) 100 233 894 97 255 891 Standard deviation 5.8 14.91 39.33 5.72 16.42 43.65 Standard deviation 5.8 14.91 39.33 5.72 16.42 43.65 CV (%) 5.8% 6.4% 4.4% 5.9% 6.4% 4.9% CV (%) 5.8% 6.4% 4.4% 5.9% 6.4% 4.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.011 0.059 0.106 0.184 0.359 0.727 1.418 2.457 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.011 0.059 0.106 0.184 0.359 0.727 1.418 2.457 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.011 0.059 0.106 0.184 0.359 0.727 1.418 2.457 O.D. 0.011 0.059 0.106 0.184 0.359 0.727 1.418 2.457

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.