Human ALCAM/CD166 ELISA Kit PicoKine®

CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 648 630 693 657 657 22.94 3.4% Sample 2 1967 2006 1987 2137 2024 66.54 3.2% Sample 3 8283 8482 8134 7570 8117 339.21 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 648 630 693 657 657 22.94 3.4% Sample 1 648 630 693 657 657 22.94 3.4% Sample 2 1967 2006 1987 2137 2024 66.54 3.2% Sample 2 1967 2006 1987 2137 2024 66.54 3.2% Sample 3 8283 8482 8134 7570 8117 339.21 4.1% Sample 3 8283 8482 8134 7570 8117 339.21 4.1%

Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 648 1967 8283 688 2156 8924 Standard deviation 37.58 145.55 563.24 48.16 198.35 651.45 CV (%) 5.8% 7.4% 6.8% 7% 9.2% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 648 1967 8283 688 2156 8924 Standard deviation 37.58 145.55 563.24 48.16 198.35 651.45 CV (%) 5.8% 7.4% 6.8% 7% 9.2% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 648 1967 8283 688 2156 8924 Mean (pg/ml) 648 1967 8283 688 2156 8924 Standard deviation 37.58 145.55 563.24 48.16 198.35 651.45 Standard deviation 37.58 145.55 563.24 48.16 198.35 651.45 CV (%) 5.8% 7.4% 6.8% 7% 9.2% 7.3% CV (%) 5.8% 7.4% 6.8% 7% 9.2% 7.3%

Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.008 0.05 0.099 0.208 0.401 0.794 1.409 2.065 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.008 0.05 0.099 0.208 0.401 0.794 1.409 2.065 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.008 0.05 0.099 0.208 0.401 0.794 1.409 2.065 O.D. 0.008 0.05 0.099 0.208 0.401 0.794 1.409 2.065

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.