Human CD200 ELISA Kit PicoKine®

Human CD200 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CD200 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 80 78 90 88 84 5.09 6% Sample 2 246 237 227 247 239 8.07 3.3% Sample 3 894 868 899 880 885 12.15 1.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 80 78 90 88 84 5.09 6% Sample 1 80 78 90 88 84 5.09 6% Sample 2 246 237 227 247 239 8.07 3.3% Sample 2 246 237 227 247 239 8.07 3.3% Sample 3 894 868 899 880 885 12.15 1.3% Sample 3 894 868 899 880 885 12.15 1.3%

Synonyms

OX-2 membrane glycoprotein; CD200; CD200; MOX1, MOX2; My033

Gene Name

CD200

Gene ID

4345

UniProt

P41217

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q31-G232

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Adaptive Immunity, Atherosclerosis, Cardiovascular, Immunology, T Cells, Vascular Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 80 246 894 73 239 980 Standard deviation 3.76 12.54 68.83 4.16 14.81 77.42 CV (%) 4.7% 7.9% 7.7% 5.7% 6.2% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 80 246 894 73 239 980 Standard deviation 3.76 12.54 68.83 4.16 14.81 77.42 CV (%) 4.7% 7.9% 7.7% 5.7% 6.2% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 80 246 894 73 239 980 Mean (pg/ml) 80 246 894 73 239 980 Standard deviation 3.76 12.54 68.83 4.16 14.81 77.42 Standard deviation 3.76 12.54 68.83 4.16 14.81 77.42 CV (%) 4.7% 7.9% 7.7% 5.7% 6.2% 7.9% CV (%) 4.7% 7.9% 7.7% 5.7% 6.2% 7.9%

Sensitivity

<10 pg/mL

Function

Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

31264 MW

Specificity

Natural and recombinant human CD200

Anticoagulant

Heparin or EDTA

Gene Name Synonym

OX-2 membrane glycoprotein

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Protein Name

OX-2 membrane glycoprotein

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.045 0.133 0.194 0.31 0.523 0.866 1.485 2.336 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.045 0.133 0.194 0.31 0.523 0.866 1.485 2.336 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.045 0.133 0.194 0.31 0.523 0.866 1.485 2.336 O.D. 0.045 0.133 0.194 0.31 0.523 0.866 1.485 2.336

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.