Human IGFBP7/Igfbp Rp1 ELISA Kit PicoKine®

Human IGFBP7/Igfbp Rp1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human IGFBP7 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Insulin-like growth factor-binding protein 7 is a protein that in humans is encoded by the IGFBP7 gene. The gene was mapped to chromosome 4q12. The major function of the protein is the regulation of availability of insulin-like growth factors (IGFs) in tissue as well as in modulating IGF binding to its receptors. IGFBP7 binds to IGF with high affinity. It also stimulates cell adhesion. The protein is implicated in some cancers.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1629 1498 1507 1608 1560 58.55 3.7% Sample 2 4265 3682 4312 3781 4010 281.18 7% Sample 3 18882 20935 19883 21797 20374 1096.22 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1629 1498 1507 1608 1560 58.55 3.7% Sample 1 1629 1498 1507 1608 1560 58.55 3.7% Sample 2 4265 3682 4312 3781 4010 281.18 7% Sample 2 4265 3682 4312 3781 4010 281.18 7% Sample 3 18882 20935 19883 21797 20374 1096.22 5.3% Sample 3 18882 20935 19883 21797 20374 1096.22 5.3%

Synonyms

Insulin-like growth factor-binding protein 7; IBP-7; IGF-binding protein 7; IGFBP-7; IGFBP-rP1; MAC25 protein; PGI2-stimulating factor; Prostacyclin-stimulating factor; Tumor-derived adhesion factor; TAF; IGFBP7; MAC25, PSF

Gene Name

IGFBP7

Gene ID

3490

UniProt

Q16270

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D30-L282

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cancer, Growth Factors/Hormones, Oncoproteins/Suppressors, Signal Transduction, Tumor Suppressors

Standard

40 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Detection Range

625 pg/mL - 40,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1629 4265 18882 1617 4427 20605 Standard deviation 114.03 319.87 1340.62 118.04 358.58 1669 CV (%) 7% 7.5% 7.1% 7.3% 8.1% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1629 4265 18882 1617 4427 20605 Standard deviation 114.03 319.87 1340.62 118.04 358.58 1669 CV (%) 7% 7.5% 7.1% 7.3% 8.1% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1629 4265 18882 1617 4427 20605 Mean (pg/ml) 1629 4265 18882 1617 4427 20605 Standard deviation 114.03 319.87 1340.62 118.04 358.58 1669 Standard deviation 114.03 319.87 1340.62 118.04 358.58 1669 CV (%) 7% 7.5% 7.1% 7.3% 8.1% 8.1% CV (%) 7% 7.5% 7.1% 7.3% 8.1% 8.1%

Sensitivity

<20 pg/mL

Function

Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

29130 MW

Specificity

Natural and recombinant human IGFBP7

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Insulin-like growth factor-binding protein 7

Subcellular Location

Secreted.

Sequence Similarities

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Protein Name

Insulin-like growth factor-binding protein 7

Sample Data

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.01 0.072 0.139 0.253 0.481 0.842 1.437 2.327 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.01 0.072 0.139 0.253 0.481 0.842 1.437 2.327 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.01 0.072 0.139 0.253 0.481 0.842 1.437 2.327 O.D. 0.01 0.072 0.139 0.253 0.481 0.842 1.437 2.327

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.