Human TNFSF14/LIGHT ELISA Kit PicoKine®

Tumor necrosis factor ligand superfamily member 14 is a protein that in humans is encoded by the TNFSF14 gene. TNFSF14 has also been designated as CD258, as well as LIGHT. It was mapped on chromosome 19p13.3. The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 71 75 65 70 70 3.56 5% Sample 2 260 268 247 270 261 9.03 3.4% Sample 3 754 758 794 885 797 52.72 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 71 75 65 70 70 3.56 5% Sample 1 71 75 65 70 70 3.56 5% Sample 2 260 268 247 270 261 9.03 3.4% Sample 2 260 268 247 270 261 9.03 3.4% Sample 3 754 758 794 885 797 52.72 6.6% Sample 3 754 758 794 885 797 52.72 6.6%

Tumor necrosis factor ligand superfamily member 14; Herpes virus entry mediator ligand; HVEM-L; Herpesvirus entry mediator ligand; CD258; Tumor necrosis factor ligand superfamily member 14, membrane form; Tumor necrosis factor ligand superfamily member 14, soluble form; TNFSF14; HVEML, LIGHT; UNQ391/PRO726

Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 71 260 754 65 241 814 Standard deviation 3.9 14.04 36.94 4.81 13.97 39.88 CV (%) 5.5% 5.4% 4.9% 7.4% 5.8% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 71 260 754 65 241 814 Standard deviation 3.9 14.04 36.94 4.81 13.97 39.88 CV (%) 5.5% 5.4% 4.9% 7.4% 5.8% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 71 260 754 65 241 814 Mean (pg/ml) 71 260 754 65 241 814 Standard deviation 3.9 14.04 36.94 4.81 13.97 39.88 Standard deviation 3.9 14.04 36.94 4.81 13.97 39.88 CV (%) 5.5% 5.4% 4.9% 7.4% 5.8% 4.9% CV (%) 5.5% 5.4% 4.9% 7.4% 5.8% 4.9%

Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.062 0.119 0.182 0.292 0.511 0.937 1.755 3.014 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.062 0.119 0.182 0.292 0.511 0.937 1.755 3.014 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.062 0.119 0.182 0.292 0.511 0.937 1.755 3.014 O.D. 0.062 0.119 0.182 0.292 0.511 0.937 1.755 3.014

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.