Human TNFRSF9/4-1BB ELISA Kit PicoKine®

CD137 is a member of the tumor necrosis factor (TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9 (TNFRSF9), 4-1BB and induced by lymphocyte activation (ILA) . CD137 gene was localized to chromosome 1p36.Members of this receptor family and their structurally related ligands are important regulators of a wide variety of physiological processes and play an especially important role in the regulation of immune responses. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 70 70 78 74 73 3.31 4.5% Sample 2 317 278 269 308 293 20.01 6.8% Sample 3 916 936 953 893 924 22.41 2.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 70 70 78 74 73 3.31 4.5% Sample 1 70 70 78 74 73 3.31 4.5% Sample 2 317 278 269 308 293 20.01 6.8% Sample 2 317 278 269 308 293 20.01 6.8% Sample 3 916 936 953 893 924 22.41 2.4% Sample 3 916 936 953 893 924 22.41 2.4%

Tumor necrosis factor receptor superfamily member 9;4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA; CD137; TNFRSF9; CD137, ILA

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 70 317 916 64 324 950 Standard deviation 7.5%5 23.45 50.38 5.44 30.13 56.05 CV (%) 7.5% 7.4% 5.5% 8.5% 9.3% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 70 317 916 64 324 950 Standard deviation 7.5%5 23.45 50.38 5.44 30.13 56.05 CV (%) 7.5% 7.4% 5.5% 8.5% 9.3% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 70 317 916 64 324 950 Mean (pg/ml) 70 317 916 64 324 950 Standard deviation 7.5%5 23.45 50.38 5.44 30.13 56.05 Standard deviation 7.5%5 23.45 50.38 5.44 30.13 56.05 CV (%) 7.5% 7.4% 5.5% 8.5% 9.3% 5.9% CV (%) 7.5% 7.4% 5.5% 8.5% 9.3% 5.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.012 0.07 0.125 0.232 0.42 0.81 1.461 2.491 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.012 0.07 0.125 0.232 0.42 0.81 1.461 2.491 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.012 0.07 0.125 0.232 0.42 0.81 1.461 2.491 O.D. 0.012 0.07 0.125 0.232 0.42 0.81 1.461 2.491

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.