Human TNFRSF9/4-1BB ELISA Kit PicoKine®

Human TNFRSF9/4-1BB ELISA Kit PicoKine® (96 Tests) . Quantitate Human TNFRSF9 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD137 is a member of the tumor necrosis factor (TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9 (TNFRSF9), 4-1BB and induced by lymphocyte activation (ILA) . CD137 gene was localized to chromosome 1p36.Members of this receptor family and their structurally related ligands are important regulators of a wide variety of physiological processes and play an especially important role in the regulation of immune responses. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 70 70 78 74 73 3.31 4.5% Sample 2 317 278 269 308 293 20.01 6.8% Sample 3 916 936 953 893 924 22.41 2.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 70 70 78 74 73 3.31 4.5% Sample 1 70 70 78 74 73 3.31 4.5% Sample 2 317 278 269 308 293 20.01 6.8% Sample 2 317 278 269 308 293 20.01 6.8% Sample 3 916 936 953 893 924 22.41 2.4% Sample 3 916 936 953 893 924 22.41 2.4%

Synonyms

Tumor necrosis factor receptor superfamily member 9;4-1BB ligand receptor; CDw137; T-cell antigen 4-1BB homolog; T-cell antigen ILA; CD137; TNFRSF9; CD137, ILA

Gene Name

TNFRSF9

Gene ID

3604

UniProt

Q07011

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L24-Q186

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed on the surface of activated T-cells.

Applications

ELISA

Field of Research

Stem Cells, T Lymphocytic Lineage, TNF Superfamily, Tumor Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 70 317 916 64 324 950 Standard deviation 7.5%5 23.45 50.38 5.44 30.13 56.05 CV (%) 7.5% 7.4% 5.5% 8.5% 9.3% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 70 317 916 64 324 950 Standard deviation 7.5%5 23.45 50.38 5.44 30.13 56.05 CV (%) 7.5% 7.4% 5.5% 8.5% 9.3% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 70 317 916 64 324 950 Mean (pg/ml) 70 317 916 64 324 950 Standard deviation 7.5%5 23.45 50.38 5.44 30.13 56.05 Standard deviation 7.5%5 23.45 50.38 5.44 30.13 56.05 CV (%) 7.5% 7.4% 5.5% 8.5% 9.3% 5.9% CV (%) 7.5% 7.4% 5.5% 8.5% 9.3% 5.9%

Sensitivity

<10 pg/mL

Function

Receptor for TNFSF9/4-1BBL. Possibly active during T cell activation.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

27899 MW

Specificity

Natural and recombinant human TNFRSF9

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Tumor necrosis factor receptor superfamily member 9

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 4 TNFR-Cys repeats.

Protein Name

Tumor necrosis factor receptor superfamily member 9

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.012 0.07 0.125 0.232 0.42 0.81 1.461 2.491 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.012 0.07 0.125 0.232 0.42 0.81 1.461 2.491 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.012 0.07 0.125 0.232 0.42 0.81 1.461 2.491 O.D. 0.012 0.07 0.125 0.232 0.42 0.81 1.461 2.491

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.