Human TNFRSF13B/TACI ELISA Kit PicoKine®

Tumor necrosis factor receptor superfamily member 13B, also known as TNFRSF13B or more commonly as TACI, is a transmembrane receptor protein found predominantly on the surface of B cells, which are an important part of the immune system. TACI controls T cell-independent B cell antibody responses, isotype switching, and B cell homeostasis. TACI is a lymphocyte-specific member of the tumor necrosis factor (TNF) receptor superfamily. It was originally discovered because of its ability to interact with calcium-modulator and cyclophilin ligand (CAML) . TACI was later found to play a crucial role in humoral immunity by interacting with two members of the TNF family: BAFF and APRIL. These proteins signal through TACI inducing activation of several transcription factors including NFAT, AP-1, and NF-kappa-B which then modulate cellular activities. Defects in the function of TACI can lead to immune system diseases.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 180 184 190 173 181 6.17 3.4% Sample 2 907 789 831 815 835 43.91 5.2% Sample 3 2596 3026 2636 2763 2755 168.03 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 180 184 190 173 181 6.17 3.4% Sample 1 180 184 190 173 181 6.17 3.4% Sample 2 907 789 831 815 835 43.91 5.2% Sample 2 907 789 831 815 835 43.91 5.2% Sample 3 2596 3026 2636 2763 2755 168.03 6% Sample 3 2596 3026 2636 2763 2755 168.03 6%

Highly expressed in spleen, thymus, small intestine and peripheral blood leukocytes. Expressed in resting B- cells and activated T-cells, but not in resting T-cells.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 180 907 2596 162 875 2578 Standard deviation 13.68 39 134.99 13.12 48.12 167.57 CV (%) 7.6% 4.3% 5.2% 8.1% 5.5% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 180 907 2596 162 875 2578 Standard deviation 13.68 39 134.99 13.12 48.12 167.57 CV (%) 7.6% 4.3% 5.2% 8.1% 5.5% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 180 907 2596 162 875 2578 Mean (pg/ml) 180 907 2596 162 875 2578 Standard deviation 13.68 39 134.99 13.12 48.12 167.57 Standard deviation 13.68 39 134.99 13.12 48.12 167.57 CV (%) 7.6% 4.3% 5.2% 8.1% 5.5% 6.5% CV (%) 7.6% 4.3% 5.2% 8.1% 5.5% 6.5%

Receptor for TNFSF13/APRIL and TNFSF13B/TALL1/BAFF/BLYS that binds both ligands with similar high affinity. Mediates calcineurin-dependent activation of NF-AT, as well as activation of NF-kappa-B and AP-1. Involved in the stimulation of B- and T- cell function and the regulation of humoral immunity. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.038 0.121 0.212 0.41 0.746 1.231 1.625 1.866 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.038 0.121 0.212 0.41 0.746 1.231 1.625 1.866 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.038 0.121 0.212 0.41 0.746 1.231 1.625 1.866 O.D. 0.038 0.121 0.212 0.41 0.746 1.231 1.625 1.866

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.