Human Periostin/OSF2 ELISA Kit PicoKine®

Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 188 183 213 194 194 11.36 5.8% Sample 2 1707 1857 1800 1793 1789 53.58 2.9% Sample 3 4824 4787 4733 4555 4724 103.2 2.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 188 183 213 194 194 11.36 5.8% Sample 1 188 183 213 194 194 11.36 5.8% Sample 2 1707 1857 1800 1793 1789 53.58 2.9% Sample 2 1707 1857 1800 1793 1789 53.58 2.9% Sample 3 4824 4787 4733 4555 4724 103.2 2.1% Sample 3 4824 4787 4733 4555 4724 103.2 2.1%

Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. .

Atherosclerosis, Cancer, Cardiogenesis, Cardiovascular, Cell Adhesion Molecules, Cell Type Markers, Cytoskeleton/ECM, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction, Structures, Tags & Cell Markers, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 188 1707 4824 180 1664 4867 Standard deviation 8.64 124.61 192.96 11.34 136.44 199.54 CV (%) 4.6% 7.3% 4% 8.2% 8.2% 4.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 188 1707 4824 180 1664 4867 Standard deviation 8.64 124.61 192.96 11.34 136.44 199.54 CV (%) 4.6% 7.3% 4% 8.2% 8.2% 4.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 188 1707 4824 180 1664 4867 Mean (pg/ml) 188 1707 4824 180 1664 4867 Standard deviation 8.64 124.61 192.96 11.34 136.44 199.54 Standard deviation 8.64 124.61 192.96 11.34 136.44 199.54 CV (%) 4.6% 7.3% 4% 8.2% 8.2% 4.1% CV (%) 4.6% 7.3% 4% 8.2% 8.2% 4.1%

Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity) . Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.078 0.128 0.25 0.442 0.853 1.434 2.097 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.078 0.128 0.25 0.442 0.853 1.434 2.097 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.078 0.128 0.25 0.442 0.853 1.434 2.097 O.D. 0.016 0.078 0.128 0.25 0.442 0.853 1.434 2.097

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.