Human IL-17RC ELISA Kit PicoKine®

Interleukin-17 receptor C is a protein that in humans is encoded by the IL17RC gene. By genomic sequence analysis, IL17RC gene was mapped to chromosome 3p25.3-p24.1. This gene encodes a single-pass transmembrane protein that shares limited similarity with the interleukin-17 receptor. Multiple alternatively spliced transcript variants encoding different isoforms have been detected for this gene, but the full-length nature of only three have been determined to date. The biologic activity of IL-17 is dependent on a complex composed of IL-17RA and IL-17RC, suggesting a new paradigm for understanding the interactions between the expanded family of IL-17 ligands and their receptors.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 343 379 386 353 365 17.78 4.8% Sample 2 2996 3084 2676 2811 2891 158.82 5.4% Sample 3 7791 7695 8440 8396 8080 339.55 4.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 343 379 386 353 365 17.78 4.8% Sample 1 343 379 386 353 365 17.78 4.8% Sample 2 2996 3084 2676 2811 2891 158.82 5.4% Sample 2 2996 3084 2676 2811 2891 158.82 5.4% Sample 3 7791 7695 8440 8396 8080 339.55 4.2% Sample 3 7791 7695 8440 8396 8080 339.55 4.2%

Interleukin-17 receptor C; IL-17 receptor C; IL-17RC; Interleukin-17 receptor homolog; IL17Rhom; Interleukin-17 receptor-like protein; IL-17RL; ZcytoR14; IL17RC; UNQ6118/PRO20040/PRO38901

Expressed in brain, cartilage, colon, heart, intestine, kidney, liver, lung, muscle, placenta, and prostate. Low expression in thymus and leukocytes. Expressed (at protein level) in prostate and prostate cancer, skeletal muscle, kidney and placenta. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 343 2996 7791 360 3053 7459 Standard deviation 24.69 224.7 420.71 32.76 267.51 499.75 CV (%) 7.2% 7.5% 5.4% 9.1% 8.7% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 343 2996 7791 360 3053 7459 Standard deviation 24.69 224.7 420.71 32.76 267.51 499.75 CV (%) 7.2% 7.5% 5.4% 9.1% 8.7% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 343 2996 7791 360 3053 7459 Mean (pg/ml) 343 2996 7791 360 3053 7459 Standard deviation 24.69 224.7 420.71 32.76 267.51 499.75 Standard deviation 24.69 224.7 420.71 32.76 267.51 499.75 CV (%) 7.2% 7.5% 5.4% 9.1% 8.7% 6.7% CV (%) 7.2% 7.5% 5.4% 9.1% 8.7% 6.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 250 500 1000 2000 4000 8000 16000 O.D. 0.006 0.064 0.125 0.259 0.511 0.936 1.623 2.094 Concentration (pg/ml) 0 250 500 1000 2000 4000 8000 16000 O.D. 0.006 0.064 0.125 0.259 0.511 0.936 1.623 2.094 Concentration (pg/ml) 0 250 500 1000 2000 4000 8000 16000 Concentration (pg/ml) 0 250 500 1000 2000 4000 8000 16000 O.D. 0.006 0.064 0.125 0.259 0.511 0.936 1.623 2.094 O.D. 0.006 0.064 0.125 0.259 0.511 0.936 1.623 2.094

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.