Rat TGF-Beta 2 ELISA Kit PicoKine®

Transforming growth factor-beta 2 (TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma (POAG) . Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured human anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 55 54 54 55 1.22 2.2% Sample 2 207 189 219 204 204 10.68 5.2% Sample 3 866 820 926 906 879 40.58 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 55 54 54 55 1.22 2.2% Sample 1 57 55 54 54 55 1.22 2.2% Sample 2 207 189 219 204 204 10.68 5.2% Sample 2 207 189 219 204 204 10.68 5.2% Sample 3 866 820 926 906 879 40.58 4.6% Sample 3 866 820 926 906 879 40.58 4.6%

Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Developmental Biology, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Metabolism, Metabolism Processes, Organogenesis, Pathways and Processes, Response To Hypoxia, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, TGF Beta

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 207 866 54 209 856 Standard deviation 2.62 15.52 39.83 2.53 16.92 42.8 CV (%) 4.6% 7.5% 4.6% 4.7% 8.1% 5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 207 866 54 209 856 Standard deviation 2.62 15.52 39.83 2.53 16.92 42.8 CV (%) 4.6% 7.5% 4.6% 4.7% 8.1% 5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 57 207 866 54 209 856 Mean (pg/ml) 57 207 866 54 209 856 Standard deviation 2.62 15.52 39.83 2.53 16.92 42.8 Standard deviation 2.62 15.52 39.83 2.53 16.92 42.8 CV (%) 4.6% 7.5% 4.6% 4.7% 8.1% 5% CV (%) 4.6% 7.5% 4.6% 4.7% 8.1% 5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.021 0.087 0.141 0.248 0.434 0.789 1.407 2.279 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.021 0.087 0.141 0.248 0.434 0.789 1.407 2.279 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.021 0.087 0.141 0.248 0.434 0.789 1.407 2.279 O.D. 0.021 0.087 0.141 0.248 0.434 0.789 1.407 2.279

Activate the sample Cell culture supernatants: add activating reagent pro rata, i.e. add 20μl of Solution A into 100μl of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6. Serum, plasma (heparin, EDTA or citrate) : add activating reagent pro rata, i.e. add 20μl of Solution A into 40μl of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6. It is unnecessary to activate the recombinant TGF-beta 2. Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration. Activate the sample Cell culture supernatants: add activating reagent pro rata, i.e. add 20μl of Solution A into 100μl of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6. Serum, plasma (heparin, EDTA or citrate) : add activating reagent pro rata, i.e. add 20μl of Solution A into 40μl of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6. It is unnecessary to activate the recombinant TGF-beta 2. Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.