Human Betacellulin/BTC ELISA Kit PicoKine®

Human Betacellulin/BTC ELISA Kit PicoKine® (96 Tests) . Quantitate Human BTC in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Betacellulin, also known as BTC is a protein that in humans is encoded by the BTC gene. This gene is mapped to 4q13.3. The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. It is the growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. This protein is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 37 37 33 35 1.63 4.6% Sample 2 220 251 215 249 233 16.36 7% Sample 3 402 461 474 465 450 28.39 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 37 37 33 35 1.63 4.6% Sample 1 36 37 37 33 35 1.63 4.6% Sample 2 220 251 215 249 233 16.36 7% Sample 2 220 251 215 249 233 16.36 7% Sample 3 402 461 474 465 450 28.39 6.3% Sample 3 402 461 474 465 450 28.39 6.3%

Synonyms

Probetacellulin; Betacellulin; BTC; BTC

Gene Name

BTC

Gene ID

685

UniProt

P35070

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D32-Y111

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Synthesized in several tissues and tumor cells. Predominantly expressed in pancreas and small intestine. .

Applications

ELISA

Field of Research

Cancer, Growth Factors, Growth Factors/Hormones, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 220 402 34 202 396 Standard deviation 1.47 12.76 26.13 1.66 17.8%5 30.88 CV (%) 4.1% 5.8% 6.5% 4.9% 7.5% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 220 402 34 202 396 Standard deviation 1.47 12.76 26.13 1.66 17.8%5 30.88 CV (%) 4.1% 5.8% 6.5% 4.9% 7.5% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 36 220 402 34 202 396 Mean (pg/ml) 36 220 402 34 202 396 Standard deviation 1.47 12.76 26.13 1.66 17.8%5 30.88 Standard deviation 1.47 12.76 26.13 1.66 17.8%5 30.88 CV (%) 4.1% 5.8% 6.5% 4.9% 7.5% 7.8% CV (%) 4.1% 5.8% 6.5% 4.9% 7.5% 7.8%

Sensitivity

<10 pg/mL

Function

Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

19746 MW

Specificity

Natural and recombinant human Betacellulin

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Probetacellulin

Subcellular Location

Betacellulin: Secreted, extracellular space.

Protein Name

Probetacellulin

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.087 0.159 0.313 0.541 0.913 1.508 2.266 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.087 0.159 0.313 0.541 0.913 1.508 2.266 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.087 0.159 0.313 0.541 0.913 1.508 2.266 O.D. 0.007 0.087 0.159 0.313 0.541 0.913 1.508 2.266

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.