Human IL-31/Interleukin-31 ELISA Kit PicoKine®

Human IL-31 ELISA Kit PicoKine® (96 Tests) . Quantitate Human IL31 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin-31, also known as IL-31 is a protein that in humans is encoded by the IL31 gene. This gene is mapped to 12q24.31. IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA and OSMR that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma. IL31 may function in skin immunity.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 134 122 129 116 125 6.83 5.4% Sample 2 804 765 827 741 784 33.38 4.2% Sample 3 1907 1624 1623 1803 1739 121.45 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 134 122 129 116 125 6.83 5.4% Sample 1 134 122 129 116 125 6.83 5.4% Sample 2 804 765 827 741 784 33.38 4.2% Sample 2 804 765 827 741 784 33.38 4.2% Sample 3 1907 1624 1623 1803 1739 121.45 6.9% Sample 3 1907 1624 1623 1803 1739 121.45 6.9%

Synonyms

Interleukin-31; IL-31; IL31

Gene Name

IL31

Gene ID

386653

UniProt

Q6EBC2

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S24-T164

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. .

Applications

ELISA

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 134 804 1907 140 740 1811 Standard deviation 8.04 44.22 122.04 10.5 52.54 130.39 CV (%) 6% 5.5% 6.4% 7.5% 7.1% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 134 804 1907 140 740 1811 Standard deviation 8.04 44.22 122.04 10.5 52.54 130.39 CV (%) 6% 5.5% 6.4% 7.5% 7.1% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 134 804 1907 140 740 1811 Mean (pg/ml) 134 804 1907 140 740 1811 Standard deviation 8.04 44.22 122.04 10.5 52.54 130.39 Standard deviation 8.04 44.22 122.04 10.5 52.54 130.39 CV (%) 6% 5.5% 6.4% 7.5% 7.1% 7.2% CV (%) 6% 5.5% 6.4% 7.5% 7.1% 7.2%

Sensitivity

<10 pg/mL

Function

Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

18205 MW

Specificity

Natural and recombinant human IL-31

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Interleukin-31

Subcellular Location

Secreted .

Protein Name

Interleukin-31

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.137 0.21 0.357 0.676 1.174 1.733 2.333 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.137 0.21 0.357 0.676 1.174 1.733 2.333 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.137 0.21 0.357 0.676 1.174 1.733 2.333 O.D. 0.055 0.137 0.21 0.357 0.676 1.174 1.733 2.333

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.