Rat CRP/C Reactive Protein ELISA Kit PicoKine®

C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 225 211 199 209 211 9.27 4.3% Sample 2 1505 1663 1572 1511 1562 63.53 4% Sample 3 4812 4672 4766 4677 4731 59.54 1.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 225 211 199 209 211 9.27 4.3% Sample 1 225 211 199 209 211 9.27 4.3% Sample 2 1505 1663 1572 1511 1562 63.53 4% Sample 2 1505 1663 1572 1511 1562 63.53 4% Sample 3 4812 4672 4766 4677 4731 59.54 1.2% Sample 3 4812 4672 4766 4677 4731 59.54 1.2%

Apoptosis, Atherosclerosis, Cardiovascular, Chromatin Binding Proteins, Complement, DNA/RNA Binding, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Macrophage/Inflammation, Metabolism, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 225 1505 4812 235 1505 4457 Standard deviation 11.02 81.27 307.96 11.75 108.36 329.81 CV (%) 4.9% 5.4% 6.4% 5% 7.2% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 225 1505 4812 235 1505 4457 Standard deviation 11.02 81.27 307.96 11.75 108.36 329.81 CV (%) 4.9% 5.4% 6.4% 5% 7.2% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 225 1505 4812 235 1505 4457 Mean (pg/ml) 225 1505 4812 235 1505 4457 Standard deviation 11.02 81.27 307.96 11.75 108.36 329.81 Standard deviation 11.02 81.27 307.96 11.75 108.36 329.81 CV (%) 4.9% 5.4% 6.4% 5% 7.2% 7.4% CV (%) 4.9% 5.4% 6.4% 5% 7.2% 7.4%

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.076 0.11 0.141 0.205 0.319 0.539 0.962 1.78 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.076 0.11 0.141 0.205 0.319 0.539 0.962 1.78 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.076 0.11 0.141 0.205 0.319 0.539 0.962 1.78 O.D. 0.076 0.11 0.141 0.205 0.319 0.539 0.962 1.78

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.